A Receptor Protein-Based Bioassay for Quantitative Determination of Paclitaxel

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• 作者：Shin-Ichiro Suye ; Sagun Tandel ; and Ashok Mulchandani
• 刊名：Analytical Chemistry
• 出版年：1997
• 出版时间：September 1, 1997
• 年：1997
• 卷：69
• 期：17
• 页码：3633 - 3635
• 全文大小：111K
• 年卷期：v.69,no.17(September 1, 1997)
• ISSN：1520-6882

文摘

A novel receptor-based bioassay for the quantitativemeasurement of Taxol was developed. The assay wasbased on the well-investigated and established findingthatTaxol, its active analogs, and active metabolites bindreversibly to the receptor protein tubulin, a processsimilar to antibody and antigen interaction. The assaywasperformed in a competitive format by allowing a mixtureof horseradish peroxidase-labeled Taxol and Taxol in theanalyte sample to compete for the Taxol binding site of apolystyrene microtiter plate wall coated with purifiedtubulin and subsequently measuring the tubulin-Taxolcomplex by determining the activity of the horseradishperoxidase label. Using this method, Taxol wasmeasuredvery sensitively, linear range of 0.0001-1 nM, andselectively, without interference from non-tumor-activecompounds such as baccatin III, cephalomaninne, and10-deacetyl taxol. The method was applied for the determination of picomolar concentrations of Taxol in human plasma.