Dual Stimuli-Responsive Vesicular Nanospheres Fabricated by Lipopolymer Hybrids for Tumor-Targeted Photodynamic Therapy

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• 作者：Johnson V. John ; Chung-Wook Chung ; Renjith P. Johnson ; Young-Il Jeong ; Kyu-Don Chung ; Dae Hwan Kang ; Hongsuk Suh ; Hongyu Chen ; Il Kim
• 刊名：Biomacromolecules
• 出版年：2016
• 出版时间：January 11, 2016
• 年：2016
• 卷：17
• 期：1
• 页码：20-31
• 全文大小：698K
• ISSN：1526-4602

文摘

Smart delivery system of photosensitizer chlorin e6 (Ce6) has been developed for targeted photodynamic therapy (PDT). Simple self-assemblies of the mixtures comprising soybean lecithin derived phosphatidylcholine (PC), phosphatidylethanolamine-poly(l-histidine)₄₀ (PE–p(His)₄₀), and folic acid (FA) conjugated phosphatidylethanolamine-poly(N-isopropylacrylamide)₄₀ (PE–p(NIPAM)₄₀–FA) in different ratios yield smart nanospheres characterized by (i) stable and uniform particle size (～100 nm), (ii) positive surface charge, (iii) high hydrophobic drug (Ce6) loading efficiency up to 45%, (iv) covalently linked targeting moiety, (v) low cytotoxicity, and (vi) smartness showing p(His) block oriented pH and p(NIPAM) oriented temperature responsiveness. The Ce6-encapsulated vesicular nanospheres (Ce6@VNS) were used to confirm the efficiency of cellular uptake, intracellular distribution, and phototoxicity against KB tumor cells compared to free Ce6 at different temperature and pH conditions. The Ce6@VNS system showed significant photodynamic therapeutic efficiency on KB cells than free Ce6. A receptor-mediated inhibition study proved the site-specific delivery of Ce6 in targeted tumor cells.