Water-Soluble Genistin Glycoside Isoflavones Up-Regulate Antioxidant Metallothionein Expression and Scavenge Free Radicals

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• 作者：Mi Ja Chung ; Ah-Young Kang ; Kyung Min Lee ; Eunji Oh ; Hee-Jin Jun ; Sang-Yeon Kim ; Joong Hyuck Auh ; Tae-Wha Moon ; Sung-Joon Lee ; and Kwan-Hwa Park
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：May 31, 2006
• 年：2006
• 卷：54
• 期：11
• 页码：3819 - 3826
• 全文大小：446K
• 年卷期：v.54,no.11(May 31, 2006)
• ISSN：1520-5118

文摘

Genistin has antioxidant activities; however, its insolubility in water often limits its biological availabilityin vivo. Using a novel transglycosylation process, the solubility of genistin glycosides was increased1000 to 10000-fold, but it was not known whether these modified genistin glycosides maintainedantioxidant activity. We found that both genistin and its glycosides similarly up-regulated thetranscription of several metallothionein (MT) antioxidant genes (MT1A, MT2A, MT1E, and MT1X),as well as the glucose 6-phosphate dehydrogenase (G6PD) gene in HepG2 cells. This gene inductionwas mediated by the sequestration of zinc in the cytosol, which up-regulated the metal-responsivetranscription factor-1 (MTF-1) that induced MT gene expression. Although not as effective as ascorbicacid, genistin glycosides possessed slightly greater reducing power than genistin. We concludedthat genistin and genistin glycosides have a direct antioxidant effect and an indirect antioxidant effect,perhaps via induction of MT by activity of MTF-1.Keywords: Genistin; genistin glycosides; metallothioneins; metal-responsive transcription factor-1