Effect of Bisphenol A on Rat Metabolic Profiling Studied by Using Capillary Electrophoresis Time-of-Flight Mass Spectrometry

详细信息    查看全文

• 作者：Jun Zeng ; Hua Kuang ; Chunxiu Hu ; Xianzhe Shi ; Min Yan ; Liguang Xu ; Libing Wang ; Chuanlai Xu ; Guowang Xu
• 刊名：Environmental Science & Technology (ES&T)
• 出版年：2013
• 出版时间：July 2, 2013
• 年：2013
• 卷：47
• 期：13
• 页码：7457-7465
• 全文大小：481K
• 年卷期：v.47,no.13(July 2, 2013)
• ISSN：1520-5851

文摘

Bisphenol A (BPA), a chemical widely used in the manufacture of polycarbonate plastics, has raised considerable concern in recent decades because of its hormone-like properties. Whether BPA exposure is a health risk remains controversial in many countries. A metabolomics study based on capillary electrophoresis time-of-flight mass spectrometry (CE-TOF/MS) was performed to study the urine metabolic profiles of Sprague鈥揇awley rats fed with four dose levels of BPA (0, 1, 10, and 100 渭g/kg body weight) for 45 days. Multivariate pattern recognition directly reflected the metabolic perturbations caused by BPA. On the basis of univariate analysis, 42 metabolites including amino acids, polyamines, nucleosides, organic acids, carbohydrates, pterins, polyphenols, and sugar phosphates were found as the most significantly differential metabolites. The marked perturbations were related with valine, leucine and isoleucine biosynthesis, d-glutamine and d-glutamate metabolism, etc. Significant alterations of neurotransmitters (glutamate, gamma-aminobutyric acid, and noradrenaline) and neurotransmitter-related metabolites (tyrosine, histamine, valine, and taurine) suggested that the toxic effects of small-dose BPA (below 50 mg/kg/day) may contribute to its interactions with the neuromediating system. Our study demonstrated that metabolomics may offer more specific insights into the molecular changes underlying the physiological effects of BPA.