Laccase Biosensor Based on Phytic Acid Modification of Nanostructured SiO2 Surface for Sensitive Detection of Dopamine

详细信息    查看全文

• 作者：Wenbo Zhao ; Kuai Wang ; Yuan Wei ; Yinghui Ma ; Lingling Liu ; Xiaohua Huang
• 刊名：Langmuir
• 出版年：2014
• 出版时间：September 23, 2014
• 年：2014
• 卷：30
• 期：37
• 页码：11131-11137
• 全文大小：475K
• ISSN：1520-5827

文摘

In this work, three kinds of nanostructured silica鈥損hytic acid (SiO₂鈥揚A) materials with diverse morphologies including spherical SiO₂鈥揚A (s-SiO₂鈥揚A), rod-like (r-SiO₂鈥揚A), and helical SiO₂鈥揚A (h-SiO₂鈥揚A) were prepared with the help of electrostatic interaction. The SiO₂鈥揚A nanomaterials with different morphologies were characterized by using transmission electron microscopy (TEM), Fourier transform infrared (FTIR), electrochemical impedance spectroscopy (EIS), and circular dichroism spectrum (CD). Diverse morphologies of SiO₂鈥揚A were used as electrode decorated materials to achieve a high efficiency for electrochemical dopamine (DA) detection. The laccase biosensors were fabricated by immobilizing different morphologies of SiO₂鈥揚A nanomaterials and laccase onto the glassy carbon electrode (GCE) surface, successively. Then the electrochemical responses of the different morphologies of nanostructured SiO₂鈥揚A nanomaterials to laccase were discussed. Results indicated that compared to laccase/s-SiO₂鈥揚A and laccase/r-SiO₂鈥揚A, the laccase/h-SiO₂鈥揚A-modified electrode showed the best electrochemical performances.