Engineering Bacteria to Form a Biofilm and Induce Clumping in Caenorhabditis elegans
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- 作者：Pedro Dorado-Morales ; Alba Iglesias ; Guillermo Zafrilla ; Alejandro Valero ; Alejandro Torres ; Samuel Miravet-Verde ; Jessica de Loma ; Marina Ma帽as ; Antonio Ruiz ; Alba Corman ; Lucas J. Morales ; Juli Peret贸 ; Cristina Vilanova ; Manuel Porcar
- 刊名：ACS Synthetic Biology
- 出版年：2014
- 出版时间：December 19, 2014
- 年：2014
- 卷：3
- 期：12
- 页码：941-943
- 全文大小：256K
- ISSN：2161-5063
文摘
Bacteria are needed for a vast range of biotechnological processes, which they carry out either as pure cultures or in association with other bacteria and/or fungi. The potential of bacteria as biofactories is hampered, though, by their limited mobility in solid or semisolid media such as agricultural or domestic waste. This work represents an attempt toward overcoming this limitation by associating bacterial biotechnological properties with the transport ability of the nematode Caenorhabditis elegans. We report here biofilm formation on C. elegans by engineered Escherichia coli expressing a Xhenorhabdus nematophila adhesion operon and induction of nematode social feeding behavior (clumping) through an E. coli-mediated iRNA blocking on the expression of FLP-21, a neuropeptide involved in worm solitary behavior.