TAML Activator-Based Amperometric Analytical Devices as Alternatives to Peroxidase Biosensors
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- 作者：Alexander D. Ryabov ; Ricardo Cer贸n-Camacho ; Omar Saavedra-D铆az ; Matthew A. Denardo ; Anindya Ghosh ; Ronan Le Lagadec ; Terrence J. Collins
- 刊名：Analytical Chemistry
- 出版年：2012
- 出版时间：November 6, 2012
- 年：2012
- 卷：84
- 期：21
- 页码：9096-9100
- 全文大小：276K
- 年卷期：v.84,no.21(November 6, 2012)
- ISSN：1520-6882
文摘
The ferric TAML catalysts [Fe{C6H2-1,2-(NCOCMe2NCO)2CMe2}(OH2)]鈭?/sup> (1) with counterions Na+ (a) and PPh4+ (b) function similar to horseradish peroxidase in the mediated electron transfer relays, which constitute a basis for amperometric biosensors. The mediators are mono- and bis-cyclometalated Ru and Os compounds of the type of [M(CN)x(NN)3-x]m+ with x = 1 and 2 (NN = 2,2鈥?bipyridine, 鈥?/sup>CN = 2-phenylpyridinato). Cyclic voltammograms of the Ru and Os compounds are not affected by 1a though cathodic currents increase drastically in the presence of hydrogen peroxide. The reduction potentials of [M(CN)x(NN)3-x]m+ complexes vary with both the nature of metal (Ru or Os) and the number of cyclometalated ligands x (1 or 2) and therefore the potential of working electrode can be set in the range of from 鈭?.1 to +0.6 V versus the normal hydrogen electrode (NHE). A prototype of a biosensor for H2O2 is described, in which the 1b catalyst and [Os(CN)2(NN)]+ mediator were coimmobilized on the surface of the glassy carbon electrode using a polymeric coating.
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