Bodipy-Labeled Nucleoside Triphosphates for Polymerase Synthesis of Fluorescent DNA

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• 作者：Dmytro Dziuba ; Radek Pohl ; Michal Hocek
• 刊名：Bioconjugate Chemistry
• 出版年：2014
• 出版时间：November 19, 2014
• 年：2014
• 卷：25
• 期：11
• 页码：1984-1995
• 全文大小：473K
• ISSN：1520-4812

文摘

New fluorescent nucleosides and nucleoside triphosphate (dNTPs) analogs bearing the F-Bodipy fluorophore linked through a short, flexible nonconjugate tether were synthesized. The Bodipy-labeled dNTPs were substrates for several DNA polymerases which incorporated them into DNA in primer extension, nicking enzyme amplification reaction, and polymerase chain reaction. The fluorescence of F-Bodipy is not quenched upon incorporation in DNA and can be detected both in solutions and on gels.