Cellular Uptake of a Fluorescent Calix[4]arene Derivative
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- 作者：Ruth Lalor ; Hugo Baillie-Johnson ; Carl Redshaw ; Susan E. Matthews ; Anja Mueller
- 刊名：Journal of the American Chemical Society
- 出版年：2008
- 出版时间：March 12, 2008
- 年：2008
- 卷：130
- 期：10
- 页码：2892 - 2893
- 全文大小：69K
- 年卷期：v.130,no.10(March 12, 2008)
- ISSN：1520-5126
文摘
A rapid synthetic route to a nontoxic fluorescently labeled water-soluble calixarene has been developed. Investigation of the cellular uptake of the labeled calixarene, via confocal microscopy, through coincubation with uptake inhibitors demonstrates that uptake is not through the common clathrin coated pits or caveolae (lipid raft) endocytic pathways and that the calixarene derivative localizes within the cytoplasm and does not enter the nucleus. The study demonstrates the power of fluorescent labeling for investigation of interactions between calixarenes and biological systems and the potential for calixarene based intracellular imaging agents.