Isolation of Lignan and Fatty Acid Derivatives from the Grains of Echinochloa utilis and Their Inhibition of Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Cells

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• 作者：Duc Hung Nguyen ; Bing Tian Zhao ; Duc Dat Le ; Young Ho Yoon ; Jee Youn Ko ; Koan Sik Woo ; Do Youn Jun ; Young Ho Kim ; Mi Hee Woo
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：January 20, 2016
• 年：2016
• 卷：64
• 期：2
• 页码：425-432
• 全文大小：375K
• ISSN：1520-5118

文摘

Two new fatty acid derivatives, echinochlorins A (8) and B (9) and a racemic lignan, (±)-anti-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-acetoxypropen-1-yl]-2-methoxyphenoxy}propan-1,3-diol 3-acetate (1), were isolated from Echinochloa utilis grains, along with six known lignans (2–7) and two fatty acid derivatives (10, 11). Their structures were established by spectroscopic data analyses (IR, UV, HR-FABMS, GC-MS, and 1D and 2D NMR). The configuration of 1 was determined by Mosher’s method. Compound 5 displayed potential inhibitory activity on lipopolysaccharide-induced NO production in macrophage RAW 264.7 cells with an IC₅₀ value of 4.8 ± 0.5 μM. These isolated compounds in crude EtOH extract were also quantitated by HPLC.