Simultaneous and Accurate Real-Time Monitoring of Glucose and Ethanol in Alcoholic Drinks, Must, and Biomass by a Dual-Amperometric Biosensor

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• 作者：Annalisa Mentana ; Carmen Palermo ; Donatella Nardiello ; Maurizio Quinto ; Diego Centonze
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：January 9, 2013
• 年：2013
• 卷：61
• 期：1
• 页码：61-68
• 全文大小：315K
• 年卷期：v.61,no.1(January 9, 2013)
• ISSN：1520-5118

文摘

In this work the optimization and application of a dual-amperometric biosensor for simultaneous monitoring of glucose and ethanol content, as quality markers in drinks and alcoholic fermentation media, are described. The biosensor is based on glucose oxidase (GOD) and alcohol oxidase (AOD) immobilized by co-cross-linking with bovine serum albumin (BSA) and glutaraldehyde (GLU) both onto a dual gold electrode, modified with a permselective overoxidized polypyrrole film (PPYox). Response, rejection of interferents, and stability of the dual biosensor were optimized in terms of PPYox thickness, BSA, and enzyme loading. The biosensor was integrated in a flow injection system coupled with an at-line microdialysis fiber as a sampling tool. Flow rates inside and outside the fiber were optimized in terms of linear responses (0.01鈥? and 0.01鈥?.5 M) and sensitivities (27.6 卤 0.4 and 31.0 卤 0.6 渭A路M^鈥?路cm^鈥?) for glucose and ethanol. Excellent anti-interference characteristics, the total absence of 鈥渃ross-talk鈥? and good response stability under operational conditions allowed application of the dual biosensor in accurate real-time monitoring (at least 15 samples/h) of alcoholic drinks, white grape must, and woody biomass.

Keywords:

dual-amperometric biosensor; glucose; ethanol; microdialysis; alcoholic drink; must; biomass