Hyperproduction of 尾-Glucanase Exg1 Promotes the Bioconversion of Mogrosides in Saccharomyces cerevisiae Mutants Defective in Mannoprotein Deposition

详细信息    查看全文

• 作者：Reuben Wang ; Pei-Yin Lin ; Shyue-Tsong Huang ; Chun-Hui Chiu ; Ting-Jang Lu ; Yi-Chen Lo
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：December 2, 2015
• 年：2015
• 卷：63
• 期：47
• 页码：10271-10279
• 全文大小：487K
• ISSN：1520-5118

文摘

Bacteria and fungi can secrete extracellular enzymes to convert macromolecules into smaller units. Hyperproduction of extracellular enzymes is often associated with alterations in cell wall structure in fungi. Recently, we identified that Saccharomyces cerevisiae kre6螖 mutants can efficiently convert mogroside V into mogroside III E, which has antidiabetic properties. However, the underlying efficient bioconversion mechanism is unclear. In the present study, the mogroside (MG) bioconversion properties of several cell wall structure defective mutants were analyzed. We also compared the cell walls of these mutants by transmission electron microscopy, a zymolyase sensitivity test, and a mannoprotein release assay. We found zymolyase-sensitive mutants (including kre1螖, las21螖, gas1螖, and kre6螖), with defects in mannoprotein deposition, exhibit efficient MG conversion and excessive leakage of Exg1; such defects were not observed in wild-type cells, or mutants with abnormal levels of glucans in the cell wall. Thus, yeast mutants defective in mannoprotein deposition may be employed to convert glycosylated bioactive compounds.