Transcriptional Profiling and Molecular Characterization of Astragalosides, Calycosin, and Calycosin-7-O-尾-d-glucoside Biosynthesis in the Hairy Roots of Astra

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• 作者：Pham Anh Tuan ; Eunsook Chung ; Aye Aye Thwe ; Xiaohua Li ; Yeon Bok Kim ; Valan Arasu Mariadhas ; Naif Abdullah Al-Dhabi ; Jai-Heon Lee ; Sang Un Park
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 15, 2015
• 年：2015
• 卷：63
• 期：27
• 页码：6231-6240
• 全文大小：418K
• ISSN：1520-5118

文摘

We used the next-generation Illumina/Solexa HiSeq2000 platform on RNA analysis to investigate the transcriptome of Astragalus membranaceus hairy roots in response to 100 渭M methyl jasmonate (MeJA). In total, 77鈥?58鈥?30 clean reads were assembled into 48鈥?36 transcripts (average length of 1398 bp), which were clustered into 23鈥?58 loci (genes). Of these, 19鈥?40 genes were annotated by BLASTx searches. In addition, DESeq analysis showed that 2127 genes were up-regulated, while 1247 genes were down-regulated by MeJA. Seventeen novel astragaloside (AST) biosynthetic genes and seven novel calycosin and calycosin-7-O-尾-d-glucoside (CG) biosynthetic genes were isolated. The accumulation of ASTs, calycosin, and CG increased significantly in MeJA-treated hairy roots compared with control hairy roots. Our findings will provide a valuable resource for molecular characterization of AST, calycosin, and CG biosynthetic pathways and may lead to new approaches to maximize their production and biomass productivity in the hairy roots of A. membranaceus.