Multiplex Lateral Flow Immunoassay for Mycotoxin Determination

详细信息    查看全文

• 作者：Suquan Song ; Na Liu ; Zhiyong Zhao ; Emmanuel Njumbe Ediage ; Songling Wu ; Changpo Sun ; Sarah De Saeger ; Aibo Wu
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：May 20, 2014
• 年：2014
• 卷：86
• 期：10
• 页码：4995-5001
• 全文大小：261K
• 年卷期：v.86,no.10(May 20, 2014)
• ISSN：1520-6882

文摘

A new lateral flow immunoassay (LFA) is proposed for qualitative and/or semiquantitative determination of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and their analogues (AFs, ZEAs, DONs) in cereal samples. Each of the mycotoxin specific antibody was class specific and there was no cross reactivity to other groups of compounds. The visual limits of detection (vLOD) of the strip were 0.03, 1.6, and 10 渭g/kg for AFB1, ZEA and DON, respectively. The calculated limits of detection (cLOD) were 0.05, 1, and 3 渭g/kg, respectively. Meanwhile the cutoff values were achieved at 1, 50, and 60 渭g/kg for AFB1, ZEA and DON, respectively. Recoveries ranged from 80% to 122% and RSD from 5% to 20%. Both the vLOD and cLOD for the three mycotoxins were lower than the EU maximum levels. Analysis of naturally contaminated maize samples resulted in a good agreement between the multiplex LFA and LC鈥揗S/MS (100% for DONs and AFs, and 81% for ZEAs). Careful analysis of the results further explained the general overestimation of LFA compared to chromatographic methods for quantification of mycotoxins.