Homogeneous Electrochemical Assay for Protein Kinase Activity

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• 作者：Ik-Soo Shin ; Rohit Chand ; Sang Wook Lee ; Hyun-Woo Rhee ; Yong-Sang Kim ; Jong-In Hong
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：November 18, 2014
• 年：2014
• 卷：86
• 期：22
• 页码：10992-10995
• 全文大小：259K
• ISSN：1520-6882

文摘

Herein, we report a homogeneous assay for protein kinase activity using an electrochemistry-based probe. The approach involves a peptide substrate conjugated with a redox tag and the phosphate-specific receptor immobilized on an electrode surface. The peptide substrate phosphorylated by a protein kinase binds to the receptor site of the probe, which results in a redox current under voltammetric measurement. Our method was successfully applied even in the presence of citrated human blood and modified to enable a single-use, chip-based electrochemical assay for kinase activity.