Combined Electrospray Ionization-Atmospheric Pressure Chemical Ionization Source for Use in High-Throughput LC-MS Applications

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• 作者：Richard T. Gallagher ; Michael P. Balogh ; Paul Davey ; Mike R. Jackson ; Ian Sinclair ; and Lisa J. Southern
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：February 15, 2003
• 年：2003
• 卷：75
• 期：4
• 页码：973 - 977
• 全文大小：104K
• 年卷期：v.75,no.4(February 15, 2003)
• ISSN：1520-6882

文摘

Fast and accurate analytical methods are essential to keeppace with sample libraries produced from combinationalchemistry and high-throughput biological screening. Manylaboratories now use a combination of ionization techniques for the characterization of these samples, includingatmospheric pressure chemical ionization (APCI), electrospray ionization (ESI), and photoionization (PI). Dataare shown here from the analysis of a compound collection plate containing a variety of sample structures. ESIwill normally analyze around 80% of these samples,necessitating a source change to analyze a further 10%.In this work, we have developed a new combined ESI-APCI source (ESCi) for use in on-line HPLC applications.The combined source allows alternate on-line ESI andAPCI scans with polarity switching within a single analysis. The ESCi source has been designed to be a simplereplacement for the existing mass spectrometer interfaces.Each ionization method is optimized independently usingseparate tuning parameters. Instrument electronics canreadily switch between the two ionization methods andpolarities within normal interscan time periods. The newsource has reduced the analysis time of sample plates byeliminating the need for a source hardware change, sourceoptimization, and repeat analyses.