HPLC Analysis of Ecdysteroids in Plant Extracts Using Superheated Deuterium Oxide with Multiple On-Line Spectroscopic Analysis (UV, IR, 1H NMR, and MS)
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- 作者：Dave Louden ; Alan Handley ; Rene Lafont ; Steve Taylor ; Ian Sinclair ; Eva Lenz ; Timothy Orton ; and Ian D. Wilson
- 刊名：Analytical Chemistry
- 出版年：2002
- 出版时间：January 1, 2002
- 年：2002
- 卷：74
- 期：1
- 页码：288 - 294
- 全文大小：108K
- 年卷期：v.74,no.1(January 1, 2002)
- ISSN：1520-6882
文摘
HPLC, using superheated D2O as the mobile phase,combined with on-line characterization via a combinationof diode array UV, 1H NMR, FT-IR spectroscopy, andmass spectrometry has been used for the analysis of astandard of 20-hydroxyecdysone- and ecdysteroid-containing plant extracts. This combination of spectrometersenabled the on-flow collection of UV, 1H NMR, IR, andmass spectra not only for pure 20-hydroxyecdysone(100-400 
g on column) but also the major ecdysteroidspresent in crude extracts of Silene otites, Silene nutans,and Silene frivaldiskyana. The ecdysteroids unequivocally identified in these extracts included 20-hydroxyecdysone, polypodine B, and integristerone A.
g on column) but also the major ecdysteroidspresent in crude extracts of Silene otites, Silene nutans,and Silene frivaldiskyana. The ecdysteroids unequivocally identified in these extracts included 20-hydroxyecdysone, polypodine B, and integristerone A.