Isolation and Determination of Anthocyanins in Seed Coats of Black Soybean (Glycine max (L.) Merr.)

详细信息    查看全文

• 作者：Myoung-Gun Choung ; In-Youl Baek ; Sung-Taeg Kang ; Won-Young Han ; Doo-Chull Shin ; Huhn-Pal Moon ; and Kwang-Hee Kang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2001
• 出版时间：December 2001
• 年：2001
• 卷：49
• 期：12
• 页码：5848 - 5851
• 全文大小：50K
• 年卷期：v.49,no.12(December 2001)
• ISSN：1520-5118

文摘

Anthocyanin pigments in seed coats of black soybean (Glycine max (L.) Merr.) were extracted with1% HCl-CH₃OH, and the crude anthocyanin extract was purified by Shepadex LH-20 and LichroprepRP-18 open-column chromatography. Three major anthocyanins were isolated, and their chemicalstructures were identified by spectroscopic methods (UV-visible, FABMS, ¹H and ¹³C NMR, andby TLC). The complete structures of these anthocyanins were elucidated as delphinidin-3-glucoside,cyanidin-3-glucoside, and petunidin-3-glucoside. Among them, petunidin-3-glucoside was identifiedas a new anthocyanin in black soybeans. On the basis of RP-HPLC with a UV-vis detector, thecontents of delphinidin-3-glucoside, cyanidin-3-glucoside, petunidin-3-glucoside, and total anthocyanins in seed coats of 10 black soybeans were found in the ranges of 0-3.71, 0.94-15.98, 0-1.41,and 1.58-20.18 mg/g, respectively. The results obtained in this study imply that the seed coats ofblack soybean can be used as a good source for cyanidin-3-glucoside and delphinidin-3-glucoside.Keywords: Black soybean; Glycine max; anthocyanins; petunidin-3-glucoside