Analysis of Pirlimycin Residues in Beef Muscle, Milk, and Honey by a Biotin–Streptavidin-Amplified Enzyme-Linked Immunosorbent Assay

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• 作者：Wenxiao Jiang ; Ross C. Beier ; Pengjie Luo ; Peng Zhai ; Nan Wu ; Guimiao Lin ; Xiaomei Wang ; Gaixia Xu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：January 13, 2016
• 年：2016
• 卷：64
• 期：1
• 页码：364-370
• 全文大小：390K
• ISSN：1520-5118

文摘

Food contamination by veterinary drug residues is a worldwide public health concern and requires continuous monitoring. In this study, we developed a biotin–streptavidin-amplified ELISA (BA-ELISA) using a produced monoclonal antibody for detecting pirlimycin residues in beef muscle, milk, and honey. The IC₅₀ value of the BA-ELISA was 1.6 ng/mL for pirlimycin in buffer, and the sensitivity was improved 3 times compared to traditional ELISAs. The optimized BA-ELISA can be used to quantitate trace amounts of pirlimycin residues in beef muscle, milk, and honey. This method had limits of detection (LODs) of 4.45 μg/kg in beef muscle, 1.65 μg/L in milk, and 2.75 μg/kg in honey. The average recovery of the BA-ELISA ranged from 78 to 97%, and the coefficient of variation ranged from 5.3 to 13.5%. The developed BA-ELISA method was validated using LC-MS/MS, and the BA-ELISA can be used for routine screening analysis of pirlimycin residues.