Seven legume extracts containing p
hytoestrogens were analyzed for estrogenic activity. Met
hanolextracts were prepared from soybean (
Glycine max L.), green bean (
Phaseolus vulgaris L.), alfalfasprout (
Medicago sativa L.), mung bean sprout (
Vigna radiata L.), kudzu root (
Pueraria lobata L.),and red clover blossom and red clover sprout (
Trifolium pratense L.). Extracts of kudzu root and redclover blossom s
howed significant competitive binding to estrogen receptor
hars/beta2.gif" BORDER=0 ALIGN="middle"> (ER
hars/beta2.gif" BORDER=0 ALIGN="middle">). Estrogenic activitywas determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzuroot, red clover blossom and sprout, mung bean sprout, and alfalfa sprout extracts displayed increasedcell proliferation above levels observed wit
h estradiol. T
he pure estrogen antagonist, ICI 182,780,suppressed cell proliferation induced by t
he extracts, suggesting an ER-related signaling pat
hwaywas involved. T
he ER subtype-selective activities of legume extracts were examined using transientlytransfected
human embryonic kidney (HEK 293) cells. All seven of t
he extracts ex
hibited preferentialagonist activity toward ER
hars/beta2.gif" BORDER=0 ALIGN="middle">. Using HPLC to collect fractions and MCF-7 cell proliferation, t
he activecomponents in kudzu root extract were determined to be t
he isoflavones puerarin, daidzin, genistin,daidzein, and genistein. T
hese results s
how t
hat several legumes are a source of p
hytoestrogenswit
h hig
h levels of estrogenic activity.Keywords: Legume; MCF-7; estrogen; flavonoid; isoflavonoid; p
hytoestrogens; estrogen receptor(
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