Attachment and Phospholipase A2-Induced Lysis of Phospholipid Bilayer Vesicles to Plasma-Polymerized Maleic Anhydride/SiO2 Multilayers
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- 作者：Anye N. Chifen ; Renate Fö ; rch ; Wolfgang Knoll ; Petra J. Cameron ; Hwei L. Khor ; Thomas L. Williams ; A. Toby A. Jenkins
- 刊名：Langmuir
- 出版年：2007
- 出版时间：May 22, 2007
- 年：2007
- 卷：23
- 期：11
- 页码：6294 - 6298
- 全文大小：233K
- 年卷期：v.23,no.11(May 22, 2007)
- ISSN：1520-5827
文摘
This article describes a method by which intact vesicles can be chemically attached to hydrolyzed maleic anhydridefilms covalently bound to plasma-polymerized SiO2 on Au substrates. Surface plasmon field-enhanced fluorescencespectroscopy (SPFS) combined with surface plasmon resonance spectroscopy (SPR) was used to monitor the activationof plasma-deposited maleic anhydride (pp-MA) film with EDC/NHS and the subsequent coupling of lipid vesicles.The vesicles were formed from a mixture of phosphatidylcholine and phosphatidylethanolamine lipids, with a water-soluble fluorophore encapsulated within. Vesicle attachment was measured in real time on plasma films formed underdifferent pulse conditions (plasma duty cycle). Optimum vesicle attachment was observed on the pp-MA films containingthe highest density of maleic anhydride groups. Phospholipase A2 was used to lyse the surface-bound vesicles andto release the encapsulated fluorophore.