Efficient Separation and Analysis of Triacylglycerols: Quantitation of 尾-Palmitate (OPO) in Oils and Infant Formulas

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• 作者：Mikhail Vyssotski ; Stephen J. Bloor ; Kirill Lagutin ; Herbert Wong ; D. Bradley G. Williams
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 8, 2015
• 年：2015
• 卷：63
• 期：26
• 页码：5985-5992
• 全文大小：295K
• ISSN：1520-5118

文摘

A high-efficiency, convenient, and reliable method for the separation of structurally similar triacylglycerols is detailed and applied in the quantitative analysis of 1,3-dioleoyl-2-palmitoylglycerol (OPO) in infant formulas and OPO oils. OPO is an important lipid component in 鈥渉umanized鈥?infant formula. A fast preparative isolation of an OPO-containing fraction from the crude complex mixture, by nonaqueous reversed phase HPLC, followed by Ag⁺-HPLC with detection at 205 nm allowed fine separation and detection of the desired fraction. OPO was quantitated independently of its regioisomer 1,2-dioleoyl-3-palmitoylglycerol (OOP) and isomers of stearoyl-linoleoyl-palmitoyl glycerol that might be present in infant formulas. For samples with low OPO content, an evaporative light-scattering detector (ELSD) was more preferable than UV detection, with a calculated LOD of 0.1 渭g of OPO injected and LOQ of 0.3 渭g. The method, which showed high reproducibility (RSD < 5%), was suitable for both high OPO content oils and low OPO products such as unenriched infant formula. A number of possible interference issues were considered and dealt with.