Polyvinyl Pyrrolidone Promotes DNA Cleavage by a ROS-Independent and Depurination Mechanism
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- 作者：Maoyong Song ; Luzhe Zeng ; Xianjun Hong ; Zihui Meng ; Junfa Yin ; Hailin Wang ; Yong Liang ; Guibin Jiang
- 刊名：Environmental Science & Technology (ES&T)
- 出版年：2013
- 出版时间：March 19, 2013
- 年：2013
- 卷：47
- 期：6
- 页码：2886-2891
- 全文大小：318K
- 年卷期：v.47,no.6(March 19, 2013)
- ISSN：1520-5851
文摘
Polyvinyl pyrrolidone polymer (PVP) has been widely applied in biological and medical fields. A few in vitro studies indicated that PVP might cause toxicity. However, the underlying mechanism is poorly understood. In this work, we found that PVP directly induced strand breakages of various DNA molecules, implicating a cleavage activity. Moreover, reactive oxygen species (ROS) scavenging analysis shows that DNA cleavage activity of PVP is not related to ROS-induced oxidation. As revealed by gel electrophoresis and liquid chromatography/mass spectrometry analysis, the major cleavage products of DNA were identified as two purine bases, guanine and adenine, suggesting that PVPs have a novel depurination activity. The selective depurination and DNA cleavage activity of PVPs were further confirmed by studying the interaction of PVP with four nucleosides and four well-designed oligodeoxynucleotides probes containing specific nucleotides. This study may provide insights into PVP-DNA interactions and resultant genotoxicity and may also open a new way for DNA study.