Stretch鈥揝towage鈥揋rowth Strategy to Fabricate Tunable Triply-Amplified Electrochemiluminescence Immunosensor for Ultrasensitive Detection of Pseudorabies Virus Antibody

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• 作者：Kang Shao ; Jing Wang ; Xiaochun Jiang ; Feng Shao ; Tingting Li ; Shiyi Ye ; Lu Chen ; Heyou Han
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：June 17, 2014
• 年：2014
• 卷：86
• 期：12
• 页码：5749-5757
• 全文大小：602K
• 年卷期：v.86,no.12(June 17, 2014)
• ISSN：1520-6882

文摘

Triply amplified electrochemical biosensors have attracted particular attention in the detection of low-abundance biomarkers. The universal construction routes for nonenzymatic triply amplified and even multiply amplified biosensors are extremely desirable but remain challenging. Here, we proposed a 鈥渟tretch鈥搒towage鈥揼rowth鈥?strategy to tunably fabricate a nonenzymatic triply amplified or multiply amplified electrochemiluminescence (ECL) immunosensor for ultrasensitive determining pseudorabies virus (PrV) antibody. Based on the matrix role of gold nanoparticle-graphene nanosheet (Au鈥揋N) hybrids, carrier role of silicon nanoparticles (SNPs) and bridge role of 鈥渂iotin鈥搒treptavidin鈥揵iotin鈥?(B鈥揝A鈥揃) structure, the establishment processes were defined as 鈥渟tretch鈥? 鈥渟towage鈥? and 鈥済rowth鈥? respectively. Relying on the interaction of antigen鈥揳ntibody and of B-SA, the 鈥淎u鈥揋N/PrV (Ag)/PrV antibody (Ab₁)/biotinylated IgG (B-Ab₂)/SA/biotinylated Ru(bpy)₃²⁺-encapsulated SNPs (B鈥揜u@SNPs)鈥?triply amplified biosensor could be fabricated and exhibited better analytical performance not only toward monoclonal PrV antibody with a linear detection range from 50 ng mL^鈥? to 1 pg mL^鈥? and a detection limit of 0.40 pg mL^鈥?, but also toward actual serum samples when compared with enzyme-linked immunosorbent assay and fluorometry. Furthermore, multiply amplified biosensors could be conveniently fabricated by controllably repeating the combination of B鈥揜u@SNPs and SA to form the B鈥揝A鈥揃 structure. After it was repeated three times, the multiply amplified biosensor stretched to the maximum of signal amplification and achieved a luminescence quantum efficiency about 23.1-fold higher than the triply amplified biosensor. The tunable biosensor exhibits good stability, acceptable reproducibility and accuracy, suggesting its potential applications in clinical diagnostics.