Characterization of Genes for a Putative Hydroxycinnamoyl-coenzyme A Quinate Transferase and p-Coumarate 3鈥?Hydroxylase and Chlorogenic Acid Accumulation in Tartary Buckwheat

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• 作者：Yeon Bok Kim ; Aye Aye Thwe ; Ye Ji Kim ; Xiaohua Li ; Haeng Hoon Kim ; Phun Bum Park ; Tastsuro Suzuki ; Sun-Ju Kim ; Sang Un Park
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：May 1, 2013
• 年：2013
• 卷：61
• 期：17
• 页码：4120-4126
• 全文大小：299K
• 年卷期：v.61,no.17(May 1, 2013)
• ISSN：1520-5118

文摘

Tartary buckwheat (Fagopyrum tataricum Gaertn.) contains a high level of flavonoid compounds, which have beneficial and pharmacological effects on health. In this study, we isolated full-length cDNAs encoding hydroxycinnamoyl-coenzyme A quinate hydroxycinnamoyltransferase (HQT) and p-coumarate 3鈥?hydroxylase (C3H), which are involved in chlorogenic acid (CGA) biosynthesis. We examined the expression levels of HQT and C3H using real-time RT-PCR in different organs and sprouts of two tartary buckwheat cultivars (Hokkai T8 and T10) and analyzed CGA content using high-performance liquid chromatography. Among the organs, the flowers in both cultivars showed the highest levels of CGA. We concluded that the expression pattern of FtHQT and FtC3H did not match the accumulation pattern of CGA in different organs of T8 and T10 cultivars. Gene expression and CGA content varied between the cultivars. We presume that FtHQT and FtC3H levels might be controlled by multiple metabolic pathways in different organs of tartary buckwheat. Probably, FtC3H might have a greater effect on CGA biosynthesis than FtHQT. Our results will be helpful for a greater understanding of CGA biosynthesis in tartary buckwheat.

Keywords:

chlorogenic acid; gene expression; light; tartary buckwheat