Preparative LC Isolation and Purification of Fumonisin B1 from Rice Culture

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• 作者：Filmore I. Meredith ; Charles W. Bacon ; Ronald D. Plattner ; and William P. Norred
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1996
• 出版时间：January 1996
• 年：1996
• 卷：44
• 期：1
• 页码：195 - 198
• 全文大小：146K
• 年卷期：v.44,no.1(January 1996)
• ISSN：1520-5118

文摘

Procedures are presented for the production and rapid isolation offumonisin B₁ (FB₁) from fumonisinB₂ (FB₂) and fumonisin B₃(FB₃), and for the purification of the mycotoxinFB₁ from cultures ofFusarium moniliforme MRC 826 grown on rice. The toxinwas extracted from rice culture withacetonitrile:water (1:1; 5mL/g of culture material), filtered, andreduced in volume on a rotaryevaporator to remove the acetonitrile. A three-step liquidchromatographic (LC) method wasdeveloped for the isolation of FB₁, FB₂, andFB₃, and the purification of FB₁.Preparative reversed-phase LC was used to isolate and partially purify the FB₁.In the first step, the extract was appliedto a Waters Bondapak PrepPak 500 C₁₈ reversed-phasecartridge and eluted with a methanol:watergradient. Fractions containing partially purified FB₁were collected, reduced in volume, andsubjected to a second preparative LC procedure. The extract wasapplied to two Bondapak PrepPakcyano cartridges, and isocratic elution with water:0.5% pyridine wasused in the purification step.Recovery of the total FB₁ was 97% from the firstisolation step and was 93% from the secondpurification step. Analytical high-performance liquidchromatography and fast-atom bombardment/mass spectrometry were used in determining the purity of theFB₁. Recovery of the high purityFB₁ (95% or greater) was 77% or 2811 mg/3647 mg ofstarting material.Keywords: Fumonisin; FB₁; FB₂; FB₃;mycotoxin