A Coordination Complex System for Generic, Ultrafast, and Sensitive Multimode Fluorescent Staining of Biomolecules

详细信息    查看全文

• 作者：Xingqiang Liu ; Lingjuan Li ; Jingjing Sun ; Yishu Yan ; Xin Shu ; Baoqing Liu ; Wei Sha ; Hui Feng ; Sha Sun ; Jin Zhu
• 刊名：Inorganic Chemistry
• 出版年：2012
• 出版时间：January 2, 2012
• 年：2012
• 卷：51
• 期：1
• 页码：188-192
• 全文大小：270K
• 年卷期：v.51,no.1(January 2, 2012)
• ISSN：1520-510X

文摘

Gel electrophoresis staining methodologies documented thus far are largely utilized in a biomolecule context-dependent manner. We report herein the development of a generic, ultrafast, and sensitive multimode fluorescent system for the efficient identification of DNA, RNA, and proteins. Interaction between a positively charged, planar ligand-based coordination complex with partner biomolecule leads to aggregation-induced fluorescence quenching and allows for the image contrast generation within one minute. Alternatively, successive reactions of the biomolecule-loaded gel with cation and ligand, in either order of sequence, provide an equally effective staining efficacy. Image contrast reversal is accomplished through a facile washing or photobleaching procedure. The versatility in the applicable target species and signal generation modes provides a hint at the design of novel staining structures and potentially enables the high-throughput readout of biomolecules.