Podosome Formation and Development in Monocytes Restricted by the Nanoscale Spatial Distribution of ICAM1

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• 作者：Andreas S. Andersen ; Hüsnü Aslan ; Mingdong Dong ; Xingyu Jiang ; Duncan S. Sutherland
• 刊名：Nano Letters
• 出版年：2016
• 出版时间：March 9, 2016
• 年：2016
• 卷：16
• 期：3
• 页码：2114-2121
• 全文大小：462K
• ISSN：1530-6992

文摘

We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.