A Genetically Encoded FRET Sensor for Intracellular Heme

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• 作者：Yanqun Song ; Maiyun Yang ; Seraphine V. Wegner ; Jingyi Zhao ; Rongfeng Zhu ; Yun Wu ; Chuan He ; Peng R. Chen
• 刊名：ACS Chemical Biology
• 出版年：2015
• 出版时间：July 17, 2015
• 年：2015
• 卷：10
• 期：7
• 页码：1610-1615
• 全文大小：574K
• ISSN：1554-8937

文摘

Heme plays pivotal roles in various cellular processes as well as in iron homeostasis in living systems. Here, we report a genetically encoded fluorescence resonance energy transfer (FRET) sensor for selective heme imaging by employing a pair of bacterial heme transfer chaperones as the sensory components. This heme-specific probe allows spatial-temporal visualization of intracellular heme distribution within living cells.