Miniaturized Electroosmotic Pump Capable of Generating Pressures of More than 1200 Bar

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• 作者：Congying Gu ; Zhijian Jia ; Zaifang Zhu ; Chiyang He ; Wei Wang ; Aaron Morgan ; Joann J. Lu ; Shaorong Liu
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：November 6, 2012
• 年：2012
• 卷：84
• 期：21
• 页码：9609-9614
• 全文大小：358K
• 年卷期：v.84,no.21(November 6, 2012)
• ISSN：1520-6882

文摘

The pressure output of a pump cannot be increased simply by connecting several of them in series. This barrier is eliminated with the micropump developed in this work. The pump is actually an assembly of a number of fundamental pump units connected in series. The maximum pressure output of this pump assembly is directly proportional to the number of serially connected pump units. Theoretically, one can always enhance the pressure output by adding more pump units in the assembly, but in reality the upper pressure is constrained by the microtees or microunions joining the pump components. With commercially available microtees and microunions, pressures of more than 1200 bar have been achieved. We have recently experimented using open capillaries to build this pump, but many capillaries have to be utilized in parallel to produce an adequate flow to drive HPLC separations. In this paper, we synthesize polymer monoliths inside 75 渭m i.d. capillaries, use these monoliths to assemble miniaturized pumps, characterize the performance of these pumps, and employ these pumps for HPLC separations of intact proteins. By tuning the experimental parameters for monolith preparations, we obtain both negatively and positively charged submicrometer capillary channels conveniently. Each monolith in a 75 渭m i.d. capillary is equivalent to several thousands of open capillaries.