Influence of Tetra(ethylene glycol) (EG4) Substitution at the Loop Region on the Intramolecular DNA i-Motif

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• 作者：Yuhe Yang ; Yawei Sun ; Yang Yang ; Yongzheng Xing ; Tao Zhang ; Zeming Wang ; Zhongqiang Yang ; Dongsheng Liu
• 刊名：Macromolecules
• 出版年：2012
• 出版时间：March 27, 2012
• 年：2012
• 卷：45
• 期：6
• 页码：2643-2647
• 全文大小：317K
• 年卷期：v.45,no.6(March 27, 2012)
• ISSN：1520-5835

文摘

In this research, we employed tetra(ethylene glycol) (EG₄) to substitute bases at the loop region of the intramolecular DNA i-motif formed by (CCCTAA)₃CCC, and systematically studied the influence of such nonbase components on the stability and conformation of the formed structures by circular dichroism (CD), UV鈥搗is spectroscopy and gel electrophoresis. We found that with all loop bases substituted, the i-motif structure can still form. The stability of the i-motif generally got weaker with the increase of the substitution number. Substitution at different positions might lead to different topologies. The findings above demonstrate that bases at the loop region play an important role on the stability and topology of the intramolecular DNA i-motif.