IbSIMT1, a novel salt-induced methyltransferase gene from Ipomoea batatas, is involved in salt tolerance

详细信息    查看全文

• 作者：Degao Liu (1)
  Shaozhen He (1)
  Xuejin Song (1)
  Hong Zhai (1)
  Ning Liu (1)
  Dongdong Zhang (1)
  Zhitong Ren (1)
  Qingchang Liu (1)
  
  1. Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization ; Ministry of Education ; China Agricultural University ; Beijing ; 100193 ; China
  
• 关键词：IbSIMT1 ; Physiological mechanism ; S ; adenosyl ; methionine ; dependent methyltransferase ; Salt tolerance ; Sweetpotato
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：120
• 期：2
• 页码：701-715
• 全文大小：9,930 KB
• 参考文献：1. Alia, Saradhi PP, Mohanty P (1991) Proline enhances primary photochemical activities in isolated thylakoid membranes of / Brassica juncea by arresting photoinhibitory damage. Biochem Biophys Res Commun 181:1238鈥?244
  2. Alia, Saradhi PP, Mohanty P (1997) Involvement of proline in protecting thylakoid membranes against free radical-induced photodamage. J Photochem Photobiol 38:253鈥?57
  3. Alia, Mohanty P, Matysik J (2001) Effect of proline on the production of singlet oxygen. Amino Acids 21:195鈥?00
  4. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:73鈥?99 CrossRef
  5. Chen HJ, Su CT, Lin CH, Huang GJ, Lin YH (2010) Expression of sweet potato cysteine protease / SPCP2 altered developmental characteristics and stress responses in transgenic / Arabidopsis plants. J Plant Physiol 167:838鈥?47 CrossRef
  6. Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010) Abscisic acid: emergence of a core signaling network. Annu Rev Plant Biol 61:651鈥?79 CrossRef
  7. De Ronde JA, Spreeth MH, Cress WA (2000) Effect of antisense L-螖¹-pyrroline-5-carboxylate reductase transgenic soybean plants subjected to osmotic and drought stress. Plant Growth Regul 32:13鈥?6 CrossRef
  8. De Ronde JA, Cress WA, Kr眉ger GHJ, Strasser RJ, Van Staden J (2004) Photosynthetic response of transgenic soybean plants, containing an / Arabidopsis / P5CR gene, during heat and drought stress. J Plant Physiol 161:1211鈥?224 CrossRef
  9. Delauney AJ, Verma DPS (1993) Proline biosynthesis and osmoregulation in plants. Plant J 4:215鈥?23 CrossRef
  10. Deng XM, Hu W, Wei SY, Zhou SY, Zhang F, Han JP, Chen LH, Li Y, Feng JL, Fang B, Luo QC, Li SS, Liu YY, Yang GX, He GY (2013) / TaCIPK29, a CBL-interacting protein kinase gene from wheat, confers salt stress tolerance in transgenic tobacco. PLoS ONE 8:e69881 CrossRef
  11. Fan WJ, Zhang M, Zhang HX, Zhang P (2012) Improved tolerance to various abiotic stresses in transgenic sweetpotato ( / Ipomoea batatas) expressing spinach betaine aldehyde dehydrogenase. PLoS ONE 7:e37344 CrossRef
  12. Fern谩ndez-Falc贸n M, Hern谩ndez M, Alvarez CE, Borges AA (2006) Variation in nutrition along time and relative chlorophyll content of / Leucospermum cordifolium cv. 鈥楬igh Gold鈥? and their relationship with chlorotic sypmptoms. Sci Hortic 107:373鈥?79 CrossRef
  13. Fuentes S, Pires N, 脴stergaard L (2010) A clade in the / QUASIMODO2 family evolved with vascular plants and supports a role for cell wall composition in adaptation to environmental changes. Plant Mol Biol 73:605鈥?15 CrossRef
  14. Gao S, Yuan L, Zhai H, Liu CL, He SZ, Liu QC (2011) Transgenic sweetpotato plants expressing an / LOS5 gene are tolerant to salt stress. Plant Cell, Tissue Organ Cult 107:205鈥?13 CrossRef
  15. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909鈥?30 CrossRef
  16. Hare PD, Cress WA (1997) Metabolic implications of stress-induced proline accumulation in plants. Plant Growth Regul 21:79鈥?02 CrossRef
  17. He SZ, Han YF, Wang YP, Zhai H, Liu QC (2009) In vitro selection and identification of sweetpotato ( / Ipomoea batatas (L.) Lam.) plants tolerant to NaCl. Plant Cell, Tissue Organ Cult 96:69鈥?4 CrossRef
  18. Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Calif Agric Exp Stn Circ 347:1鈥?9
  19. Huang P, Ju HW, Min JH, Zhang X, Kim SH, Yang KY, Kim CS (2013) Overexpression of L-type lectin-like protein kinase 1 confers pathogen resistance and regulates salinity response in / Arabidopsis thaliana. Plant Sci 203鈥?04:98鈥?06 CrossRef
  20. Joshi R, Ramanarao MV, Lee S, Kato N, Baisakh N (2014) Ectopic expression of ADP ribosylation factor 1 ( / SaARF1) from smooth cordgrass ( / Spartina alterniflora Loisel) confers drought and salt tolerance in transgenic rice and / Arabidopsis. Plant Cell, Tissue Organ Cult 117:17鈥?0 CrossRef
  21. Kim SH, Ahn YO, Ahn MJ, Jeong JC, Lee HS, Kwak SS (2013a) Cloning and characterization of an orange gene that increases carotenoid accumulation and salt stress tolerance in transgenic sweetpotato cultures. Plant Physiol Biochem 70:445鈥?54 CrossRef
  22. Kim SH, Kim YH, Ahn YO, Ahn MJ, Jeong JC, Lee HS, Kwak SS (2013b) Down-regulation of the lycopene 系-cyclase gene increases carotenoid synthesis via the 尾-branch-specific pathway and聽enhances salt-stress tolerance in sweetpotato transgenic calli. Physiol Plant 147:432鈥?42 CrossRef
  23. Koca H, Ozdemir F, Turkan I (2006) Effect of salt stress on lipid peroxidation and superoxide dismutase and peroxidase activities of / Lycopersicon esculentum and / L. pennellii. Biol Plant 50:745鈥?48 CrossRef
  24. Krupkov谩 E, Immerzeel P, Pauly M, Schm眉lling T (2007) The / TUMOROUS SHOOT DEVELOP- / MENT2 gene of / Arabidopsis encoding a putative methyltransferase is required for cell adhesion and co-ordinated plant development. Plant J 50:735鈥?50 CrossRef
  25. Letunic I, Goodstadt L, Dickens NJ, Doerks T, Schultz J, Mott R, Ciccarelli F, Copley RR, Ponting CP, Bork P (2002) Recent improvements to the SMART domain-based sequence annotation resource. Nucleic Acids Res 30:242鈥?44 CrossRef
  26. Liu QC, Zhai H, Wang Y, Zhang DP (2001) Efficient plant regeneration from embryogenic suspension cultures of sweetpotato. In Vitro Cell Dev Biol Plant 37:564鈥?67 CrossRef
  27. Liu DG, He SZ, Zhai H, Wang LJ, Zhao Y, Wang B, Li RJ, Liu QC (2014a) Overexpression of / IbP5CR enhances salt tolerance in transgenic sweetpotato. Plant Cell, Tissue Organ Cult 117:1鈥?6 CrossRef
  28. Liu DG, Wang LJ, Liu CL, Song XJ, He SZ, Zhai H, Liu QC (2014b) An / Ipomoea batatas iron-sulfur cluster scaffold protein gene, / IbNFU1, is involved in salt tolerance. PLoS ONE 9(4):e93935 CrossRef
  29. Martin JL, McMillan FM (2002) SAM (dependent) I AM: the / S-adenosylmethionine-dependent methyltransferase fold. Curr Opin Struct Biol 12:783鈥?93 CrossRef
  30. Mayoral JG, Nouzova M, Yoshiyama M, Shinoda T, Hernandez-Martinez S, Dolghih E, Turjanski AG, Roitberg AE, Priestap H, Perez M, Mackenzie L, Li Y, Noriega FG (2009) Molecular and functional characterization of a juvenile hormone acid methyltransferase expressed in the corpora allata of mosquitoes. Insect Biochem Mol Biol 39:31鈥?7 CrossRef
  31. Miao YS, Li HY, Shen JB, Wang JQ, Jiang LW (2011) QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in / Arabidopsis suspension-cultured cells. J Exp Bot 62:5063鈥?078 CrossRef
  32. Miller G, Shulaev V, Mittler R (2008) Reactive oxygen signaling and abiotic stress. Physiol Plant 133:481鈥?89 CrossRef
  33. Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R (2010) Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant, Cell Environ 33:453鈥?67 CrossRef
  34. Mishra MK, Chaturvedi PC, Singh R, Singh G, Sharma LK, Pandey V, Kumari N, Misra P (2013) Overexpression of / WsSGTL1 gene of / Withania somnifera enhances salt tolerance, heat tolerance and cold acclimation ability in transgenic / Arabidopsis plants. PLoS ONE 8:e63064 CrossRef
  35. Mouille G, Ralet MC, Cavelier C, Eland C, Effroy D, H茅maty K, McCartney L, Truong HN, Gaudon V, Thibault JF, Marchant A, H枚fte H (2007) Homogalacturonan synthesis in / Arabidopsis thaliana requires a Golgi-localized protein with a putative methyltransferase domain. Plant J 50:605鈥?14 CrossRef
  36. Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651鈥?81 CrossRef
  37. Park SC, Song WS, Yoon S (2014) Structural analysis of a putative SAM-dependent methyltransferase, YtqB, from / Bacillus subtilis. Biochem Biophys Res Commun 446:921鈥?26 CrossRef
  38. Peng XJ, Zhang LX, Zhang LX, Liu ZJ, Cheng LQ, Yang Y, Shen SH, Chen SY, Liu GS (2013) The transcriptional factor / LcDREB2 cooperates with / LcSAMDC2 to contribute to salt tolerance in / Leymus chinensis. Plant Cell, Tissue Organ Cult 113:245鈥?56 CrossRef
  39. RoyChoudhury A, Roy C, Sengupta DN (2007) Transgenic tobacco plants overexpressing the heterologous / lea gene / Rab16A from rice during high salt and water deficit display enhanced tolerance to salinity stress. Plant Cell Rep 26:1839鈥?859 CrossRef
  40. Sahr T, Adam T, Fizames C, Maurel C, Santoni V (2010) O-carboxyl-and N-methyltransferases active on plant aquaporins. Plant Cell Physiol 51:2092鈥?104 CrossRef
  41. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3:1101鈥?108 CrossRef
  42. Schubert HL, Blumenthal RM, Cheng XD (2003) Many paths to methyltransfer: a chronicle of convergence. Trends Biochem Sci 28:329鈥?35 CrossRef
  43. Singh S, Singh C, Tripathi AK (2014) A SAM-dependent methyltransferase cotranscribed with arsenate reductase alters resistance to peptidyl transferase center-binding antibiotics in / Azospirillum brasilense Sp7. Appl Microbiol Biotechnol 98:4625鈥?636 CrossRef
  44. Smirnoff N, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28:1057鈥?060 CrossRef
  45. Struck AW, Thompson ML, Wong LS, Micklefield J (2012) / S-adenosyl-methionine-dependent methyltransferases: highly versatile enzymes in biocatalysis, biosynthesis and other biotechnological applications. ChemBioChem 13:2642鈥?655 CrossRef
  46. Surekha C, Kumari KN, Aruna LV, Suneetha G, Arundhati A, Kishor PBK (2014) Expression of the / Vigna aconitifolia P5CSF129A gene in transgenic pigeonpea enhances proline accumulation and salt tolerance. Plant Cell, Tissue Organ Cult 116:27鈥?6 CrossRef
  47. Takahashi S, Murata N (2008) How do environmental stresses accelerate photoinhibition? Trends Plant Sci 13:178鈥?82 CrossRef
  48. Wang LJ, He SZ, Zhai H, Liu DG, Wang YN, Liu QC (2013a) Molecular cloning and fanctional characterization of a salt tolerance-associated gene / IbNFU1 from sweetpotato. J Integr Agric 12:27鈥?5 CrossRef
  49. Wang TZ, Zhang JL, Tian QY, Zhao MG, Zhang WH (2013b) A / Medicago truncatula EF-Hand family gene, / MtCaMP1, is involved in drought and salt stress tolerance. PLoS ONE 8:e58952 CrossRef
  50. Wyn Jones RG, Storey R (1978) Salt stress and comparative physiology in the / Gramineae. II. Glycine betaine and proline accumulation in two salt- and water- stressed barley cultivars. Aust J Plant Physiol 5:817鈥?29 CrossRef
  51. Yang A, Dai XY, Zhang WH (2012) A R2R3-type MYB gene, / OsMYB2, is involved in salt, cold, and dehydration tolerance in rice. J Exp Bot 63:2541鈥?556 CrossRef
  52. Yang L, Han HJ, Liu MM, Zuo ZJ, Zhou KQ, L眉 JC, Zhu YR, Bai YL, Wang Y (2013) Overexpression of the / Arabidopsis photorespiratory pathway gene, serine: glyoxylate aminotransferase ( / AtAGT1), leads to salt stress tolerance in transgenic duckweed ( / Lemna minor). Plant Cell, Tissue Organ Cult 113:407鈥?16 CrossRef
  53. Zang N, Zhai H, Gao S, Chen W, He SZ, Liu QC (2009) Efficient production of transgenic plants using the / bar gene for herbicide resistance in sweetpotato. Sci Hortic 122:649鈥?53 CrossRef
  54. Zhang H, Han B, Wang T, Chen S, Li H, Zhang Y, Dai S (2012a) Mechanisms of plant salt response: insights from proteomics. J Proteome Res 11:49鈥?7 CrossRef
  55. Zhang H, Liu YX, Xu Y, Chapman S, Love AJ, Xia T (2012b) A newly isolated Na⁺/H⁺ antiporter gene, / DmNHX1, confers salt tolerance when expressed transiently in / Nicotiana benthamiana or stably in / Arabidopsis thaliana. Plant Cell, Tissue Organ Cult 110:189鈥?00 CrossRef
  56. Zhao Q, Zhang H, Wang T, Chen SX, Dai SJ (2013) Proteomics-based investigation of salt-responsive mechanisms in plant roots. J Proteom 82:230鈥?53 CrossRef
  57. Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247鈥?73 CrossRef
  58. Zsigmond L, Szepesi 脕, Tari I, Rig贸 G, Kir谩ly A, Szabados L (2012) Overexpression of the mitochondrial / PPR40 gene improves salt tolerance in / Arabidopsis. Plant Sci 182:87鈥?3 CrossRef
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

S-adenosyl-methionine (SAM)-dependent methyltransferase (MTase) genes are a multigene family; however, only a few have been characterized at the functional level. In the present study, a novel salt-induced SAM-dependent MTase gene, named IbSIMT1, was isolated from salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line ND98. IbSIMT1 contains a DUF248 domain of unknown function and an MTase domain. Expression of IbSIMT1 was up-regulated in sweetpotato under salt stress and abscisic acid treatment. The IbSIMT1-overexpressing sweetpotato (cv. Shangshu 19) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbSIMT1 up-regulated the salt stress responsive genes, including pyrroline-5-carboxylate synthase, pyrroline-5-carboxylate reductase, SOD, psbA and phosphoribulokinase genes under salt stress. These findings suggest that the novel IbSIMT1 gene is involved in sweetpotato salt tolerance and enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and increasing reactive oxygen species scavenging capacity.