Overexpression of Lhx8 inhibits cell proliferation and induces cell cycle arrest in PC12 cell line

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• 作者：Haoming Li (1)
  Jianbing Qin (1)
  Guohua Jin (1) (2)
  Linqing Zou (1) (2)
  Jinhong Shi (1) (2)
  Xiao Han (1) (2)
  Xiang Cheng (1)
  Xinhua Zhang (1)
  
  1. Department of Human Anatomy ; the Jiangsu Key Laboratory of Neuroregeneration ; Medical School of Nantong University ; 19 Qixiu Road ; Nantong ; 226001 ; Jiangsu ; China
  2. Department of Human Anatomy and Histoembryology ; Medical School of Soochow University ; Suzhou ; 215123 ; China
  
• 关键词：Lhx8 ; PC12 ; Proliferation ; Cell cycle
• 刊名：In Vitro Cellular & Developmental Biology - Animal
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：51
• 期：4
• 页码：329-335
• 全文大小：2,268 KB
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• 刊物主题：Cell Biology; Developmental Biology; Stem Cells; Cell Culture; Animal Genetics and Genomics;
• 出版者：Springer US
• ISSN：1543-706X

文摘

LIM-homeobox genes play a pivotal function in tissue patterning and differentiation, Lhx8 is a member of LIM-homeobox gene family, and it is selectively expressed in embryonic basal forebrain and is a key factor for the determination of cholinergic cells fate. However, besides cholinergic differentiation, little is known about the potential role of Lhx8 in cell biology. In this study, we transfected Lhx8 complementary DNA (cDNA) into PC12 cell line using lentiviral vectors to acquire the cells which stably expressed high level of Lhx8, and we provide the experimental evidence that overexpression of Lhx8 inhibits cell proliferation and induces cell cycle arrest but not apoptosis in vitro. In conclusion, besides cholinergic differentiation, our results suggest that Lhx8 also plays as a suppressor gene of proliferation in cell biology.