Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli
详细信息 查看全文
- 作者：Prashant Kumar Singh ; Alok Kumar Shrivastava…
- 关键词：Anabaena sp. PCC 7120 ; Escherichia coli ; ADP ; ribose pyrophosphatase ; Hypothetical protein ; Homology modeling ; Molecular docking ; Cloning and expression
- 刊名：Functional & Integrative Genomics
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：17
- 期：1
- 页码：39-52
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Cell Biology; Plant Genetics & Genomics; Microbial Genetics and Genomics; Biochemistry, general; Bioinformatics; Animal Genetics and Genomics;
- 出版者：Springer Berlin Heidelberg
- ISSN：1438-7948
- 卷排序：17
文摘
In silico derived properties on experimental validation revealed that hypothetical protein Alr2954 of Anabaena sp. PCC7120 is ADP-ribose pyrophosphatase, which belongs to nudix hydrolase superfamily. Presence of ADP-ribose binding site was attested by ADP-ribose pyrophosphatase activity (Km 44.71 ± 8.043 mM, Vmax 7.128 ± 0.417 μmol min−1 mg protein−1, and Kcat/Km 9.438 × 104 μM−1 min−1). Besides ADP-ribose, the enzyme efficiently hydrolyzed various nucleoside phosphatases such as 8-oxo-dGDP, 8-oxo-dADP, 8-oxo-dGTP, 8-oxo-dATP, GDP-mannose, ADP-glucose, and NADH. qRT-PCR analysis of alr2954 showed significant expression under different abiotic stresses reconfirming its role in stress tolerance. Thus, Alr2954 qualifies to be a member of nudix hydrolase superfamily, which serves as ADP-ribose pyrophosphatase and assists in multiple abiotic stress tolerance.