The Changes in the Expression of NF-KB in a Degenerative Human Intervertebral Disc model

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• 作者：Zhongyi Sun ; Zhanmin Yin ; Chao Liu ; Jiwei Tian
• 关键词：NF ; kB ; Intervertebral disc degeneration ; Signaling pathway ; Nucleus pulposus cell
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：72
• 期：1
• 页码：115-122
• 全文大小：927 KB
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• 作者单位：Zhongyi Sun (1)
  Zhanmin Yin (1) (2)
  Chao Liu (1)
  Jiwei Tian (1)
  
  1. Department of Orthopedics Surgery, School of Medicine, Shanghai First People’s Hospital Affiliated Shanghai Jiao Tong University, 100, Haining Road, Shanghai, 200080, China
  2. Spine and Joint Surgery, Central Hospital of Tai An, Shandong, China
  
• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283

文摘

We aim at determining the changes in the expression of NF-kB signaling pathway in degenerative intervertebral discs. We collected normal and degenerated intervertebral discs tissues. The normal and degenerated cells were cultivated and their histopathology and immunofluoresence studies were used to observe the position of NF-kB p65 in the cell. We also treated the nucleus pulposus cells with inflammatory factors and inhibitors. Western blot was used to analyze the expression of different proteins. Real time fluorescence-based quantitative PCR was used for observation of NF-kB regulation of change in gene expression. Immunofluorescence showed that in the non-degenerative group the p65 was found in the cytoplasm of the nucleus pulposus cell while in the degenerated cell group the p65 protein was found in the nucleus of the cell. The expression of p65 increased with increase in the degree of degenerative change of the nucleus pulposus cell. RT-PCR showed that the expression of matrix metalloproteinases, aggrecanases and IL-6 was higher in the degenerative group. The amount of aggrecan and type II collagen was significantly decreased in the degenerative group. IL-1β was able to upregulate the activation of NF-kB and the expression of MMP-13 and ADAMTS-4 was also significantly increased. The effect of these proteins can be inhibited by the NF-kB inhibitor, BAY11-7082. The activation of the NK-kB signaling pathway in a degenerative intervertebral disc is gradually increased, regulating the over-expression of matrix-degrading enzymes. It plays an important role in the degradation of extracellular matrix.