Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp. pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in

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• 作者：Mei-Fang Song (1) (2) (3)
  Shu Zhang (2)
  Pei Hou (3)
  Hong-Zhong Shang (1)
  Hai-Ke Gu (1)
  Jing-Juan Li (2)
  Yang Xiao (4)
  Lin Guo (3)
  Liang Su (3)
  Jian-Wei Gao (2)
  Jian-Ping Yang (3)
  
  1. Beijing Radiation Center ; Beijing ; 100875 ; People鈥檚 Republic of China
  2. Institute of Vegetables and Flowers ; Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Greenhouse Vegetable Biology ; Jinan ; 250100 ; People鈥檚 Republic of China
  3. Institute of Crop Sciences ; Chinese Academy of Agricultural Sciences ; 12 Zhongguancun South Street ; Haidian District ; Beijing ; 100081 ; People鈥檚 Republic of China
  4. Graduate School ; Chinese Academy of Agricultural Sciences ; Beijing ; 100081 ; People鈥檚 Republic of China
  
• 关键词：Arabidopsis thaliana ; Chinese cabbage ; Ectopic expression ; De ; etiolation ; Flowering time ; Phytochrome B
• 刊名：Plant Molecular Biology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：87
• 期：6
• 页码：633-643
• 全文大小：2,180 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Biochemistry
  Plant Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-5028

文摘

Phytochrome B (phyB) is an essential red light receptor that predominantly mediates seedling de-etiolation, shade-avoidance response, and flowering time. In this study, we isolate a full-length cDNA of PHYB, designated BrPHYB, from Chinese cabbage (Brassica rapa L. ssp. pekinensis), and we find that BrphyB protein has high amino acid sequence similarity and the closest evolutionary relationship to Arabidopsis thaliana phyB (i.e., AtphyB). Quantitative reverse transcription (RT)-PCR results indicate that the BrPHYB gene is ubiquitously expressed in different tissues under all light conditions. Constitutive expression of the BrPHYB gene in A. thaliana significantly enhances seedling de-etiolation under red- and white-light conditions, and causes dwarf stature in mature plants. Unexpectedly, overexpression of BrPHYB in transgenic A. thaliana resulted in reduced expression of gibberellins biosynthesis genes and delayed flowering under short-day conditions, whereas AtPHYB overexpression caused enhanced expression of FLOWERING LOCUS T and earlier flowering. Our results suggest that BrphyB might play an important role in regulating the development of Chinese cabbage. BrphyB and AtphyB have conserved functions during de-etiolation and vegetative plant growth and divergent functions in the regulation of flowering time.