One-step formation and sterilization of gellan and hyaluronan nanohydrogels using autoclave

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• 作者：Elita Montanari (1)
  Maria Cristina De Rugeriis (1)
  Chiara Di Meo (1)
  Roberta Censi (2)
  Tommasina Coviello (1)
  Franco Alhaique (1)
  Pietro Matricardi (1)
  
  1. Department of Drug Chemistry and Technologies ; 鈥淪apienza鈥?University of Rome ; P.le Aldo Moro 5 ; 00185 ; Rome ; Italy
  2. School of Pharmacy ; University of Camerino ; Via S. Agostino ; 1 ; 62032 ; Camerino ; MC ; Italy
  
• 刊名：Journal of Materials Science Materials in Medicine
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：26
• 期：1
• 全文大小：575 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biomaterials
  Characterization and Evaluation Materials
  Polymer Sciences
  Metallic Materials
  Ceramics,Glass,Composites,Natural Materials
  Surfaces and Interfaces and Thin Films
  
• 出版者：Springer Netherlands
• ISSN：1573-4838

文摘

The sterilization of nanoparticles for biomedical applications is one of the challenges that must be faced in the development of nanoparticulate systems. Usually, autoclave sterilization cannot be applied because of stability concerns when polymeric nanoparticles are involved. This paper describes an innovative method which allows to obtain, using a single step autoclave procedure, the preparation and, at the same time, the sterilization of self-assembling nanohydrogels (NHs) obtained with cholesterol-derivatized gellan and hyaluronic acid. Moreover, by using this approach, NHs, while formed in the autoclave, can be easily loaded with drugs. The obtained NHs dispersion can be lyophilized in the presence of a cryoprotectant, leading to the original NHs after re-dispersion in water.