Predatory efficiency of the copepod Megacyclops formosanus and toxic effect of the red alga Gracilaria firma-synthesized silver nanoparticles against the dengue vector Aedes aegypti

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• 作者：Kandasamy Kalimuthu ; Chellasamy Panneerselvam ; Chi Chou ; Showe-Mei Lin…
• 关键词：Gracilaria firma ; Megacyclops formosanus ; Silver nanoparticles (AgNPs) ; Aedes aegypti ; Red algae
• 刊名：Hydrobiologia
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：785
• 期：1
• 页码：359-372
• 全文大小：1,505 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5117
• 卷排序：785

文摘

Vector control is a critical requirement in epidemic disease management, and improved mosquito control methods that are economic and effective are necessary. Cyclopoid copepods are common predators in numerous aquatic ecosystems and have been used as biological agents in programs to control mosquito larvae. In this study, we proposed a green synthesis of silver nanoparticles (AgNPs) using the seaweed, Gracilaria firma, and combined them with the copepod, Megacyclops formosanus, for controlling the dengue vector, Aedes aegypti. The green synthesis of AgNPs was characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy. In mosquitocidal assays, the LC₅₀ of G. firma extract against A. aegypti ranged from 0.091 (instar I) to 2.417 (pupae). LC₅₀ of G. firma-synthesized AgNPs ranged from 25.895 (instar I) to 351.419 (pupae). Furthermore, we evaluated the predatory efficiency of copepod M. formosanus against the larvae of A. aegypti. The most characteristic effects were midgut columnar cell vacuolization, epithelium cell contents passing into the midgut lumen, and, finally, cell death. These results suggest that synthesized AgNPs in combination with copepods constitute ecofriendly and potential mosquito larvicidal agents.