Measurement of ex vivo ELISpot interferon-gamma recall responses to Plasmodium falciparum AMA1 and CSP in Ghanaian adults with natural exposure to malaria

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• 作者：Harini Ganeshan ; Kwadwo A. Kusi ; Dorothy Anum ; Michael R. Hollingdale…
• 关键词：Circumsporozoite protein ; CSP ; Apical membrane antigen ; 1 ; AMA1 ; Natural transmission ; Ghana ; Ex vivo ELISpot ; IFN ; γ ; T cells ; Class I ; restricted epitope ; Peptide ; HLA
• 刊名：Malaria Journal
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：15
• 期：1
• 全文大小：1,260 KB
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• 作者单位：Harini Ganeshan (1)
  Kwadwo A. Kusi (2)
  Dorothy Anum (2)
  Michael R. Hollingdale (1)
  Bjoern Peters (3)
  Yohan Kim (3)
  John K. A. Tetteh (2)
  Michael F. Ofori (2)
  Ben A. Gyan (2)
  Kwadwo A. Koram (2)
  Jun Huang (1)
  Maria Belmonte (1)
  Jo Glenna Banania (1)
  Daniel Dodoo (2)
  Eileen Villasante (1)
  Martha Sedegah (1)
  
  1. Malaria Department, Naval Medical Research Center, Silver Spring, MD, USA
  2. Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
  3. La Jolla Institute for Allergy and Immunology, La Jolla, San Diego, CA, USA
  
• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine;
• 出版者：BioMed Central
• ISSN：1475-2875

文摘

Background Malaria eradication requires a concerted approach involving all available control tools, and an effective vaccine would complement these efforts. An effective malaria vaccine should be able to induce protective immune responses in a genetically diverse population. Identification of immunodominant T cell epitopes will assist in determining if candidate vaccines will be immunogenic in malaria-endemic areas. This study therefore investigated whether class I-restricted T cell epitopes of two leading malaria vaccine antigens, Plasmodium falciparum circumsporozoite protein (CSP) and apical membrane antigen-1 (AMA1), could recall T cell interferon-γ responses from naturally exposed subjects using ex vivo ELISpot assays.