Protection via a ROM4 DNA vaccine and peptide against Toxoplasma gondii in BALB/c mice
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- 作者：Yali Han ; Aihua Zhou ; Gang Lu ; Guanghui Zhao ; Lin Wang…
- 关键词：Toxoplasma gondii ; Vaccine ; ROM4 ; Peptide ; Bioinformatics ; Immunization strategy
- 刊名：BMC Infectious Diseases
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：17
- 期：1
- 全文大小：847KB
- 刊物主题：Infectious Diseases; Parasitology; Medical Microbiology; Tropical Medicine; Internal Medicine;
- 出版者：BioMed Central
- ISSN：1471-2334
- 卷排序：17
文摘
BackgroundToxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite with a broad host range including most warm-blooded animals, including humans. T. gondii surface antigen 1 (SAG1) is a well-characterized T. gondii antigen. T. gondii expresses five nonmitochondrial rhomboid intramembrane proteases, TgROM1-5. TgROM4 is uniformly distributed on the surface of T. gondii and involved in regulating MIC2, MIC3, MIC6, and AMA1 during T. gondii invasion of host cells. Bioinformatics have predicted ROM4 B-cell and T-cell epitopes. Immunization strategy is also a key factor in determining the effectiveness of the immune response and has gained increasing attention in T. gondii vaccine research. In this study, we used a DNA prime-peptide boost vaccination regimen to assess the protective efficacy of various vaccination strategies using TgROM4.