Comparative measurement of CNP and NT-proCNP in human blood samples: a methodological evaluation
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- 作者：Andreas Kuehnl (1)
Jaroslav Pelisek (1)
Martin Bruckmeier (1)
Wajima Safi (1)
Hans-Henning Eckstein (1) - 关键词：CNP ; NT ; proCNP ; Sample processing
- 刊名：Journal of Negative Results in BioMedicine
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：12
- 期：1
- 全文大小：211KB
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Jaroslav Pelisek (1)
Martin Bruckmeier (1)
Wajima Safi (1)
Hans-Henning Eckstein (1)
1. Clinic for Vascular and Endovascular Surgery, Klinikum rechts der Isar, Technische Universit?t München, Ismaninger Strasse 22, Munich, 81675, Germany - ISSN：1477-5751
文摘
Background C-type natriuretic peptide (CNP) has anti-inflammatory, anti-proliferative, and anti-migratory properties. During the past years, CNP has attained an increasing interest by many research groups, especially in the cardiovascular field. Nevertheless, still no reliable data exist on the difference of CNP concentration between serum and plasma samples. Also, the influence of delayed blood sample proceeding is unknown. The aim of this study was to investigate the difference of CNP and NT-proCNP concentrations between serum and plasma samples. In order to identify potential methodological bias, this study should also validate the stability of CNP and NT-proCNP in full blood samples stored at room temperature. Findings Triplets (serum, plasma, full blood) of fasting blood samples from 12 healthy male individuals were collected. Analysis of CNP and NT-proCNP concentration was performed immediately following sampling, and after 30?minutes or 2?hours of storage at room temperature. Mean serum concentrations at baseline were 0.997?±-.379?ng/ml for CNP and 58.5?±-8.3?pg/ml for NT-proCNP. Furthermore, NT-proCNP concentration did not change significantly during the allotted time and did not differ between serum, plasma, and full blood samples. At baseline, concentrations of CNP were significantly different between samples containing either sodium-citrate or EDTA as a clotting inhibitor (1.933?±-.699?ng/ml vs. 0.991?±-.489?ng/ml, p--.001). Conclusions CNP and NT-proCNP are stable for at least two hours, even when sample processing is delayed or blood probes are stored at room temperature. NT-proCNP assay demonstrated more consistent and reliable data and should therefore be preferred for usage in clinical applications. Nevertheless, as recommended for ANP and BNP, immunoassays for CNP should also be standardized or harmonized in the future.