Subacute toxicity study of the methanolic seed extract of Buchholzia coriacea (Capparaceae) in rats

详细信息    查看全文

• 作者：Nwakaego Ernestina Nweze (1) Nwakaego_ernestina@yahoo.com
  Boniface M. Anene (1)
  Isaac U. Asuzu (2)
  Wilfred S. Ezema (3)
• 关键词：Buchholzia coriacea – ; Subacute toxicity – ; Rats – ; Blood biochemistry – ; Haematology – ; Necropsy
• 刊名：Comparative Clinical Pathology
• 出版年：2012
• 出版时间：October 2012
• 年：2012
• 卷：21
• 期：5
• 页码：967-974
• 全文大小：222.9 KB
• 参考文献：1. Ajaiyeoba EO, Onocha DA, Olarenwaju OT (2001) In vitro anthelmintic properties of Buchholzia coriacea and Gynandsopsis gynandra extracts. Pharm Biol 39:217–220
  2. Capasso R, Izzo AA, Pinto L, Bifulco T, Vitobello T, Mascolo N (2000) Phytotherapy and quality of herbal medicines. Fitoterapia 71:S58–S65
  3. Coles EH (1968) Veterinary Clinical Pathology, 3rd Edition. W. B. Saunders Co. Philadelphia, pp 145–151.
  4. Doumas BT, Watson WA, Biggs HG (1971) Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chim Acta 31:87–96
  5. Ezekiel OO, Onyeoziri NF (2009) Preliminary studies on the antimicrobial properties of Buchholzia coriacea (wonderful kola). Afr J Biotechnol 8:472–474
  6. Fawcett JK, Scott JE (1960) A rapid and precise method for the determination of urea. J Clin Pathol 13:156–159
  7. Fossati P, Prencipe L, Berti G (1983) Enzymatic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement. Clin Chem 29:1494–1496
  8. Maruna RFL (1958) Beitrag zur serum-natrium-bestimmung Eine kritische studie iiber kolorimetrische bestimmungen und angabe einer einfachen fotometrischen methode. Clin Chim Acta 2:581–585
  9. Meoni P (2008) Pharmacology and toxicology of drug discovery and development, a paper presented at a workshop organized by CSIR titled ‘Drug Design and Development’ held at the University of Pretoria, South Africa, October 27–29.
  10. Nweze NE, Asuzu IU (2006) The anthelmintic effects of Buchholzia coriacea seed. Niger Vet J 27:60–65
  11. Nweze NE, Fakae LB, Asuzu IU (2009) Trypanocidal activity of the ethanolic extract of Buchholzia coriacea seed. Niger Vet J 29:1–6
  12. Reitman S, Frankel S (1957) A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvate transaminases. Am J Clin Pathol 28:56–63
  13. Rodriguez-Fragoso L, Reyes-Esparza J, Burchiel SW, Herrera-Ruiz D, Torres E (2008) Risks and benefits of commonly used herbal medicines in Mexico. Toxicol Appl Pharmacol 227:125–135
  14. Roeschlaw P, Bernt E, Gruber JW (1974) Enzymatic determination of total cholesterol in serum. J Clin Chem Clin Biochem 12:226–403
  15. Terri AE, Sesin PG (1958) Colorimetric determination of potassium in human serum and plasma using sodium tetraphenyl boron. Am J Clin Pathol 29:86
• 作者单位：1. Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria2. Department of Veterinary Physiology & Pharmacology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria3. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
• ISSN：1618-565X

文摘

The methanol extract of Buchholzia coriacea seed has shown some anti-trypanosomal activity against Trypanosoma brucei brucei. The acute toxicity test of this extract showed no signs of toxicity. This present study was designed to assess its sub-acute toxicity in rats. Rats of both sexes were dosed daily with 125, 250 and 500 mg/kg body weight of the extract by oral gavage for 28 days. Dosing with the extract showed no significant effect on body weights. The temperatures of extract-treated rats were significantly (p < 0.05) higher during the first and last weeks of the experiment. There were also significant reductions (p < 0.05) of red and white blood cell counts, packed cell volumes and haemoglobin concentrations in the extract-treated animals. Blood biochemistry revealed no significant changes. On necropsy, pale mucous membranes, bile stains on spleen, congestion of the lungs and caudal lobes of the liver were evident in the extract-treated rats. There were significant (p < 0.05) reductions in relative liver weights of the extract-treated male rats. Twenty-eight days dosing of rats with the methanol extract of B. coriacea seed caused signs of toxicity at the tested doses.