Antithrombotic and antiplatelet activities of pelargonidin in vivo and in vitro

详细信息    查看全文

• 作者：Sae-Kwang Ku ; Eun-Kyung Yoon ; Wonhwa Lee ; Sinae Kwon…
• 关键词：Pelargonidin ; Anticoagulation ; Antiplatelets ; Fibrinolysis ; Endothelium
• 刊名：Archives of Pharmacal Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：39
• 期：3
• 页码：398-408
• 全文大小：903 KB
• 参考文献：Bae JS, Lee W, Nam JO, Kim JE, Kim SW, Kim IS (2014) Transforming growth factor beta-induced protein promotes severe vascular inflammatory responses. Am J Respir Crit Care Med 189:779–786CrossRef PubMed
  Bauer KA (2013) Pros and cons of new oral anticoagulants. Hematology Am Soc Hematol Educ Program 2013:464–470CrossRef PubMed
  Beecher GR (2003) Overview of dietary flavonoids: nomenclature, occurrence and intake. J Nutr 133:3248S–3254SPubMed
  Beretz A, Cazenave JP, Anton R (1982) Inhibition of aggregation and secretion of human platelets by quercetin and other flavonoids: structure-activity relationships. Agents Actions 12:382–387CrossRef PubMed
  Dahlback B (2000) Blood coagulation. Lancet 355:1627–1632CrossRef PubMed
  Davie EW (1995) Biochemical and molecular aspects of the coagulation cascade. Thromb Haemost 74:1–6PubMed
  Davie EW, Fujikawa K, Kisiel W (1991) The coagulation cascade: initiation, maintenance, and regulation. Biochemistry 30:10363–10370CrossRef PubMed
  Despotis GJ, Gravlee G, Filos K, Levy J (1999) Anticoagulation monitoring during cardiac surgery: a review of current and emerging techniques. Anesthesiology 91:1122–1151CrossRef PubMed
  Diehl KH, Hull R, Morton D, Pfister R, Rabemampianina Y, Smith D, Vidal JM, van de Vorstenbosch C (2001) A good practice guide to the administration of substances and removal of blood, including routes and volumes. J Appl Toxicol 21:15–23CrossRef PubMed
  Esmon CT (2005) The interactions between inflammation and coagulation. Br J Haematol 131:417–430CrossRef PubMed
  Fares A (2013) Winter cardiovascular diseases phenomenon. N Am J Med Sci 5:266–279CrossRef PubMed PubMedCentral
  Franke B, Akkerman JW, Bos JL (1997) Rapid Ca2 + -mediated activation of Rap1 in human platelets. EMBO J 16:252–259CrossRef PubMed PubMedCentral
  Fukumoto LR, Mazza G (2000) Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem 48:3597–3604CrossRef PubMed
  Furie B, Furie BC (2005) Thrombus formation in vivo. J Clin Invest 115:3355–3362CrossRef PubMed PubMedCentral
  Ghosh S, Ezban M, Persson E, Pendurthi U, Hedner U, Rao LV (2007) Activity and regulation of factor VIIa analogs with increased potency at the endothelial cell surface. J Thromb Haemost 5:336–346CrossRef PubMed
  Hamaguchi E, Takamura T, Shimizu A, Nagai Y (2003) Tumor necrosis factor-alpha and troglitazone regulate plasminogen activator inhibitor type 1 production through extracellular signal-regulated kinase- and nuclear factor-kappaB-dependent pathways in cultured human umbilical vein endothelial cells. J Pharmacol Exp Ther 307:987–994CrossRef PubMed
  Hamalainen M, Nieminen R, Vuorela P, Heinonen M, Moilanen E (2007). Anti-Inflammatory Effects of Flavonoids: Genistein, Kaempferol, Quercetin, and Daidzein Inhibit STAT-1 and NF-kB Activations, Whereas Flavone, Isorhamnetin, Naringenin, and Pelargonidin Inhibit only NF-kB Activation along with Their Inhibitory Effect on iNOS Expression and NO Production in Activated Macrophages-Volume 2007, Article ID 45673. Mediators of Inflammation
  Hidalgo M, Martin-Santamaria S, Recio I, Sanchez-Moreno C, de Pascual-Teresa B, Rimbach G, de Pascual-Teresa S (2012) Potential anti-inflammatory, anti-adhesive, anti/estrogenic, and angiotensin-converting enzyme inhibitory activities of anthocyanins and their gut metabolites. Genes Nutr 7:295–306CrossRef PubMed PubMedCentral
  Hirsh J, Raschke R, Warkentin TE, Dalen JE, Deykin D, Poller L (1995) Heparin: mechanism of action, pharmacokinetics, dosing considerations, monitoring, efficacy, and safety. Chest 108:258S–275SCrossRef PubMed
  Jauch EC, Saver JL, Adams HP Jr, Bruno A, Connors JJ, Demaerschalk BM, Khatri P, McMullan PW Jr, Qureshi AI, Rosenfield K, Scott PA, Summers DR, Wang DZ, Wintermark M, Yonas H (2013) Guidelines for the early management of patients with acute ischemic stroke: a guideline for healthcare professionals from the American Heart Association/American Stroke Association. Stroke 44:870–947CrossRef PubMed
  Kamei H, Kojima T, Hasegawa M, Koide T, Umeda T, Yukawa T, Terabe K (1995) Suppression of tumor cell growth by anthocyanins in vitro. Cancer Invest 13:590–594CrossRef PubMed
  Kamenickova A, Anzenbacherova E, Pavek P, Soshilov AA, Denison MS, Anzenbacher P, Dvorak Z (2013) Pelargonidin activates the AhR and induces CYP1A1 in primary human hepatocytes and human cancer cell lines HepG2 and LS174T. Toxicol Lett 218:253–259CrossRef PubMed PubMedCentral
  Kang SY, Seeram NP, Nair MG, Bourquin LD (2003) Tart cherry anthocyanins inhibit tumor development in Apc(Min) mice and reduce proliferation of human colon cancer cells. Cancer Lett 194:13–19CrossRef PubMed
  Katsube N, Iwashita K, Tsushida T, Yamaki K, Kobori M (2003) Induction of apoptosis in cancer cells by Bilberry (Vaccinium myrtillus) and the anthocyanins. J Agric Food Chem 51:68–75CrossRef PubMed
  K-i Oyama, Kawaguchi S, Yoshida K, Kondo T (2007) Synthesis of pelargonidin 3-O-6″-O-acetyl-β-d-glucopyranoside, an acylated anthocyanin, via the corresponding kaempferol glucoside. Tetrahedron Lett 48:6005–6009CrossRef
  Kim TH, Ku SK, Bae JS (2012) Antithrombotic and profibrinolytic activities of eckol and dieckol. J Cell Biochem 113:2877–2883CrossRef PubMed
  Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R (2003) Analysis and biological activities of anthocyanins. Phytochemistry 64:923–933CrossRef PubMed
  Ku SK, Bae JS (2014) Antiplatelet and antithrombotic activities of purpurogallin in vitro and in vivo. BMB Rep 47:376–381CrossRef PubMed PubMedCentral
  Ku SK, Han MS, Lee MY, Lee YM, Bae JS (2014) Inhibitory effects of oroxylin A on endothelial protein C receptor shedding in vitro and in vivo. BMB Rep 47:336–341CrossRef PubMed PubMedCentral
  Ku SK, Lee W, Kang M, Bae JS (2015) Antithrombotic activities of aspalathin and nothofagin via inhibiting platelet aggregation and FIIa/FXa. Arch Pharm Res 38:1080–1089CrossRef PubMed
  Lee KH (2000) Research and future trends in the pharmaceutical development of medicinal herbs from Chinese medicine. Public Health Nutr 3:515–522CrossRef PubMed
  Lee W, Ku SK, Bae JS (2015) Antiplatelet, anticoagulant, and profibrinolytic activities of baicalin. Arch Pharm Res 38:893–903CrossRef PubMed
  Lopez S, Peiretti F, Bonardo B, Juhan-Vague I, Nalbone G (2000) Effect of atorvastatin and fluvastatin on the expression of plasminogen activator inhibitor type-1 in cultured human endothelial cells. Atherosclerosis 152:359–366CrossRef PubMed
  Marin L, Miguelez EM, Villar CJ, Lombo F (2015) Bioavailability of dietary polyphenols and gut microbiota metabolism: antimicrobial properties. Biomed Res Int 2015:905215CrossRef PubMed PubMedCentral
  Noda Y, Kaneyuki T, Mori A, Packer L (2002) Antioxidant activities of pomegranate fruit extract and its anthocyanidins: delphinidin, cyanidin, and pelargonidin. J Agric Food Chem 50:166–171CrossRef PubMed
  Nowak P, Zbikowska HM, Ponczek M, Kolodziejczyk J, Wachowicz B (2007) Different vulnerability of fibrinogen subunits to oxidative/nitrative modifications induced by peroxynitrite: functional consequences. Thromb Res 121:163–174CrossRef PubMed
  Oldoni TLC, Melo PS, Massarioli AP, Moreno IAM, Bezerra RMN, Rosalen PL, da Silva GVJ, Nascimento AM, Alencar SM (2016) Bioassay-guided isolation of proanthocyanidins with antioxidant activity from peanut (Arachis hypogaea) skin by combination of chromatography techniques. Food Chem 192:306–312CrossRef PubMed
  Paredes-Lopez O, Cervantes-Ceja ML, Vigna-Perez M, Hernandez-Perez T (2010) Berries: improving human health and healthy aging, and promoting quality life–a review. Plant Foods Hum Nutr 65:299–308CrossRef PubMed
  Pereira MS, Melo FR, Mourao PA (2002) Is there a correlation between structure and anticoagulant action of sulfated galactans and sulfated fucans? Glycobiology 12:573–580CrossRef PubMed
  Philip-Joet F, Alessi MC, Philip-Joet C, Aillaud M, Barriere JR, Arnaud A, Juhan-Vague I (1995) Fibrinolytic and inflammatory processes in pleural effusions. Eur Respir J 8:1352–1356CrossRef PubMed
  Quinn C, Hill J, Hassouna H (2000) A guide for diagnosis of patients with arterial and venous thrombosis. Clin Lab Sci 13:229–238PubMed
  Rao LV, Rapaport SI, Lorenzi M (1988) Enhancement by human umbilical vein endothelial cells of factor Xa-catalyzed activation of factor VII. Blood 71:791–796PubMed
  Ruggeri ZM (2002) Platelets in atherothrombosis. Nat Med 8:1227–1234CrossRef PubMed
  Schleef RR, Bevilacqua MP, Sawdey M, Gimbrone MA Jr, Loskutoff DJ (1988) Cytokine activation of vascular endothelium. Effects on tissue-type plasminogen activator and type 1 plasminogen activator inhibitor. J Biol Chem 263:5797–5803PubMed
  Schmitt E, Stopper H (2001) Estrogenic activity of naturally occurring anthocyanidins. Nutr Cancer 41:145–149CrossRef PubMed
  Schouten M, Wiersinga WJ, Levi M, van der Poll T (2008) Inflammation, endothelium, and coagulation in sepsis. J Leukoc Biol 83:536–545CrossRef PubMed
  Sim D, Flaumenhaft R, Furie B (2005) Interactions of platelets, blood-borne tissue factor, and fibrin during arteriolar thrombus formation in vivo. Microcirculation 12:301–311CrossRef PubMed
  Sugo T, Nakamikawa C, Tanabe S, Matsuda M (1995) Activation of prothrombin by factor Xa bound to the membrane surface of human umbilical vein endothelial cells: its catalytic efficiency is similar to that of prothrombinase complex on platelets. J Biochem 117:244–250CrossRef PubMed
  Tijburg LBM, Mattern T, Folts JD, Weisgerber UM, Katan MB (1997) Tea flavonoids and cardiovascular diseases: a review. Crit Rev Food Sci Nutr 37:771–785CrossRef PubMed
  Tsuda T, Horio F, Osawa T (1998) Dietary cyanidin 3-O-beta-D-glucoside increases ex vivo oxidation resistance of serum in rats. Lipids 33:583–588CrossRef PubMed
  Wang H, Cao G, Prior RL (1997) Oxygen radical absorbing capacity of anthocyanins. J Agric Food Chem 45:304–309CrossRef
  Weitz JI, Hirsh J, Samama MM (2004) New anticoagulant drugs: the Seventh ACCP conference on antithrombotic and thrombolytic therapy. Chest 126:265S–286SCrossRef PubMed
  Wells PS, Forgie MA, Rodger MA (2014) Treatment of venous thromboembolism. JAMA 311:717–728CrossRef PubMed
  Zhu Z, Kuang M, Lamikanra O, Liu G, Li S, He J (2015) Preparation and toxicological evaluation of methyl pyranoanthocyanin. Food Chem Toxicology. 83:125–132CrossRef
  
• 作者单位：Sae-Kwang Ku (1)
  Eun-Kyung Yoon (2)
  Wonhwa Lee (2) (3)
  Sinae Kwon (2)
  Taeho Lee (2)
  Jong-Sup Bae (2)
  
  1. Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea
  2. College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea
  3. Department of Biochemistry and Cell Biology, BK21 Plus KNU Biomedical Convergence Program, School of Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea
  
• 刊物主题：Pharmacy; Pharmacology/Toxicology;
• 出版者：Springer Netherlands
• ISSN：1976-3786

文摘

Pelargonidin is a well-known red pigment found in plants, and has been reported as having important biological activities that are potentially beneficial for human health. However, the possible roles of pelargonidin as an anticoagulant and the underlying mechanism have not yet been elucidated. We tested the effect of pelargonidin and its glucoside-conjugated form, pelargonidin-3-glucoside, on the clotting times, such as activated partial thromboplastin time (aPTT) and prothrombin time (PT), and the activities and productions of thrombin and activated factor X (FXa). Furthermore, the effects of pelargonidin on the fibrin polymerization, platelet aggregation, and the ratio of plasminogen activator inhibitor-1 (PAI-1) to tissue plasminogen activator were determined. Pelargonidin, but not pelargonidin-3-glucoside, prolonged the aPTT and PT, and inhibited the activity and production of thrombin and FXa in human umbilical vein endothelial cells. Furthermore, pelargonidin inhibited thrombin-catalyzed fibrin polymerization and platelet aggregation and elicited anticoagulant effects in mice. In addition, pelargonidin significantly reduced PAI-1 to t-PA ratio. Collectively, these results indicate that the anthocyanin pelargonidin possesses antithrombotic activity, and can be beneficial in preventing thrombus formation, thus improving blood circulation.