Genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria arborescens

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• 作者：Ken Komatsu ; Yukie Katayama ; Tsutomu Omatsu ; Tetsuya Mizutani…
• 刊名：Archives of Virology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：161
• 期：9
• 页码：2627-2631
• 全文大小：417 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798
• 卷排序：161

文摘

The phytopathogenic fungus Alternaria spp. contains a variety of double-stranded RNA (dsRNA) elements of different sizes. Detailed analysis of next-generation sequencing data obtained using dsRNA purified from Alternaria arborescens, from which we had previously found Alternaria arborescens victorivirus 1, revealed the presence of another mycoviral-like dsRNA of approximately 2.5 kbp in length. When using the fungal mitochondrial genetic code, this dsRNA has a single open reading frame that potentially encodes an RNA-dependent RNA polymerase (RdRp) with significant to sequence similarity to those of viruses of the genus Mitovirus. Moreover, both the 5’- and 3’-untranslated regions have the potential to fold into stable stem-loop structures, which is characteristic of mitoviruses. Pairwise comparisons and phylogenetic analysis of the deduced amino acid sequences of RdRp indicated that the virus we identified in A. arborescens is a distinct member of the genus Mitovirus in the family Narnaviridae, designated as “Alternaria arborescens mitovirus 1” (AaMV1).Electronic supplementary materialThe online version of this article (doi:10.1007/s00705-016-2953-1) contains supplementary material, which is available to authorized users.