Expression and roles of As-NUPR1 protein from Artemia sinica during embryo development and in response to salinity stress
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- 作者：Rui Zhang (1)
Feng Yao (1)
Cheng Cheng (1)
Yifei Chen (1)
Ying Lv (1)
Zengrong Li (1)
Na Zhao (1)
Ting Wang (1)
Wenting Xin (1)
Xiangyang Zou (2)
Lin Hou (1) - 关键词：Artemia sinica ; As ; NUPR1 ; Embryo development ; Salinity stress
- 刊名：Molecular Biology Reports
- 出版年：2014
- 出版时间：May 2014
- 年：2014
- 卷：41
- 期：5
- 页码：3465-3473
- 全文大小：
- 参考文献：1. Jiang LJ, Hou L, Zou XY, Zhang RF, Wang JQ, Sun WJ (2007) Cloning and expression analysis of p26 gene in / Artemia sinica. Acta Biochim Biophys Sin 39:351-58 CrossRef
2. Hou L, Zou XY, Yao F (2005) Study on biological characteristics of / Artemia. Bull Biol 40:4-
3. Bao Y, Peng SJ (2007) Reproduction path and effect factors of brine shrimp. Mar Sci 31:81-3
4. Kishida M, Johanning KM, Bengtson DA, Specker JL (1998) Intestinal uptake of lipovitellin from brine shrimp ( / Artemia franciscana) by larval inland silversides ( / Menidia beryllina) and striped bass ( / Morone saxatilis). Comp Biochem Physiol 19A:415-21 CrossRef
5. Sugumar V, Munuswamy N (2007) Physical, biochemical and functional characterization of haemoglobin from three strains of / Artemia. Comp Biochem Physiol 146A:291-98 CrossRef
6. Zhou Q, Wu CG, Dong B, Liu FQ, Xiang JH (2008) The encysted dormant embryo proteome of / Artemia sinica. Mar Biotechnol 10:438-46 CrossRef
7. Browne RA, Sorgeloos P, Trotman CAN (eds) (1991) / Artemia biology. CRC Press, Boca Raton, pp 93-18
8. MacRae TH (2005) Diapause: diverse states of developmental and metabolic arrest. J Biol Res 3:3-4
9. MacRae TH (2010) Gene expression, metabolic regulation and stress tolerance during diapause. Cell Mol Life Sci 67:2405-424 CrossRef
10. Denlinger DL (2002) Regulation of diapause. Annu Rev Entomol 47:93-22 CrossRef
11. Lopes FL, Desmarais JA, Murphy BD (2004) Embryonic diapause and its regulation. Reproduction 128:669-78 CrossRef
12. Baumeister R, Schaffitzel E, Hertweck Mn (2006) Endocrine signaling in / Caenorhabditis elegans controls stress response and longevity. J Endocrinol 190:191-02 CrossRef
13. Kostal V (2006) Eco-physiological phases of insect diapause. J Insect Physiol 52:113-27 CrossRef
14. Nambu Z, Tanaka S, Nambu F, Nakano M (2008) Influence of temperature and darkness on embryonic diapause termination in dormant / Artemia cysts that have never been desiccated. J Exp Zool A Ecol Genet Physiol 309:17-4
15. Robbins HM, Van SG, Sorgeloos P, Sung YY, MacRae TH, Bossier P (2010) Diapause termination and development of encysted / Artemia embryos: roles for nitric oxide and hydrogen peroxide. J Exp Biol 213:1464-470 CrossRef
16. Jiang WG, Davies G, Fodstad O (2005) Com-1/P8 in oestrogen regulated growth of breast cancer cells, the ER-b connection. Biochem Biophys Res Commun 330:253-62 CrossRef
17. Mallo GV, Fiedler F, Calvo EL, Ortiz EM, Vasseur S, Keim V, Morisset J, Iovanna JL (1997) Cloning and expression of the rat p8 cDNA, a new gene activated in pancreas during the acute phase of pancreatitis, pancreatic development, and regeneration, and which promotes cellular growth. J Biol Chem 272:32360-2369 CrossRef
18. Vasseur S, Mallo GV, Fiedler F, Bodeker H, Canepa E, Moreno S, Iovanna JL (1999) Cloning and expression of human p8, a nuclear protein with mitogenic activity. Eur J Biochem 259:670-75 CrossRef
19. Vasseur S, Mallo GV, Garcia-Montero A, Ortiz EM, Fiedler F, Cainepa E, Moreno S, Iovanna JL (1999) Structural and functional characterization of the mouse p8 gene: promotion of transcription by the CAAT-enhancer binding protein alpha (C/EBPalpha) and C/EBPbeta transacting factors involves a C/EBP cis-acting element and other regions of the promoter. Biochem J 343:377-83 CrossRef
20. Igarashi T, Kuroda H, Takahashi S, Asashima M (2001) Cloning and characterization of the / Xenopus laevis p8 gene. Dev Growth Differ 43:693-98 CrossRef
21. Massari ME, Murre C (2000) Helix-loop-helix proteins: regulators of transcription in eukaryotic organisms. Mol Cell Biol 20:429-40 CrossRef
22. Jones S (2004) An overview of the basic helix-loop-helix proteins. Genome Biol 5:226 CrossRef
23. Bustin M, Reeves R (1996) High-mobility-group chromosomal proteins: architectural components that facilitate chromatin function. Prog Nucleic Acid Res Mol Biol 54:35-00 CrossRef
24. Encinar JA, Mallo GV, Mizyrycki C, Giono L, Gonzalez-Ros JM, Rico M, Canepa E, Moreno S, Neira JL, Iovanna JL (2001) Human p8 is a HMG-I/Y-like protein with DNA binding activity enhanced by phosphorylation. J Biol Chem 276:2742-751 CrossRef
25. Jiang YF, Vaccaro MI, Fiedler F, Calvo EL, Iovanna JL (1999) Lipopolysaccharides induce p8 mRNA expression in vivo and in vitro. Biochem Biophys Res Commun 260:686-90 CrossRef
26. Qiu ZJ, MacRae TH (2007) Developmentally regulated synthesis of p8, a stress-associated transcription cofactor, in diapause-destined embryos of / Artemia franciscana. Cell Stress Chaperones 12:255-64 CrossRef
27. Malicet C, Hoffmeister A, Moreno S, Closa D, Dagorn JC, Vasseur S, Iovanna JL (2006) Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation. Biochem Biophys Res Commun 339:284-89 CrossRef
28. Path GA, Opel A, Knoll A, Seufert J (2004) Nuclear protein p8 is associated with glucose-induced pancreatic beta-cell growth. Diabetes 53:S82–S85 CrossRef
29. Bratland A, Risberg K, Maelandsmo GM, Gutzkow KB, Olsen OE, Moghaddam A, Wang MY, Hansen CM, Blomhoff HK, Berg JP, Fodstad O, Ree AH (2000) Expression of a novel factor, com1, is regulated by 1, 25-dihydroxyvitamin D3 in breast cancer cells. Cancer Res 60:5578-583
30. Malicet C, Lesavre N, Vasseur S, Iovanna JL (2003) p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growth. Mol Cancer 2:37-9 CrossRef
31. Quirk CC, Seachrist DD, Nilson JH (2003) Embryonic expression of the luteinizing hormone beta gene appears to be couple to the transient appearance of p8, a high mobility group-related transcription factor. J Biol Chem 278:1680-685 CrossRef
32. Li X, Hou L, Ma J, Liu YD, Zheng LP, Zou XY (2012) Cloning and characterization of β-catenin gene in early embryonic developmental stage of / Artemia sinica. Mol Biol Rep 39:701-07 CrossRef
33. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248-54 CrossRef
34. Sch?gger H (2006) Tricine–SDS-PAGE. Nat Protoc 1:16-2 CrossRef
35. Wang JQ, Han JC, Li DZ, Li LC (2009) In silico cloning and characterization of p8 homolog cDNA from common urchin ( / Paracentrotus lividus). Mol Biol Rep 36:2431-437 CrossRef
36. Vasseur S, Folch-Puy E, Hlouschek V, Garcia S, Fiedler F, Lerch MM, Dagorn JC, Closa D, Iovanna JL (2004) p8 improves pancreatic response to acute pancreatitis by enhancing the expression of the anti-inflammatory protein pancreatitis-associated protein I. J Biol Chem 279:7199-207 CrossRef
37. Garcia-Montero AC, Vasseur S, Giono LE, Canepa E, Moreno S, Dagorn JC, Lovanna JL (2001) Transforming growth factor beta-1 enhances Smad transcriptional activity through activation of p8 gene expression. Biochem J 357:249-53 CrossRef
38. Vasseur S, Hoffmeister A, Garacia S, Bagnis C, Dagorn JC, Iovanna JL (2002) p8 is critical for tumour development induced rasv12 mutated protein and E1A oncogene. EMBO Rep 3:165-70 CrossRef
39. Kong DK, Georgescu SP, Cano C, Aronovitz MJ, Iovanna JL, Patten RD, Kyriakis JM, Goruppi S (2010) Deficiency of the transcriptional regulator p8 results in increased autophagy and apoptosis, and causes impaired heart function. Mol Biol Cell 21:1335-349 CrossRef
40. Plant SR, Wang Y, Vasseur S, Thrash JC, Mcmahon EJ, Bergstralh DT, Arnett HA, Miller SD, Carson MJ, Iovanna JL, Ting JP-Y (2006) Upregulation of the stress-associated gene p8 in mouse models of demyelination and in multiple sclerosis tissues. Glia 53:529-37 CrossRef
41. Su SB, Motoo Y, Iovanna JL, Berthezene P, Xie MJ, Mouri H, Ohtsubo K, Matsubara F, Sawabu N (2001) Overexpression of p8 is inversely correlated with apoptosis in pancreatic cancer. Clin Cancer Res 7:1320-324
42. Giroux V, Malicet C, Barthet M, Gironella M, Archange C, Dagorn JC, Vasseur S, Iovanna JL (2006) p8 is a new target of gemcitabine in pancreatic cancer cells. Clin Cancer Res 12:235-41 CrossRef
43. Liu Z, Sun Y, Liu N, Fan N, Zhang S (2009) Characterization, expression, and response to stress of p8 gene in amphioxus. Fish Shellfish Immunol 27:407-13 CrossRef
44. Zheng LP, Hou L, Chang AK, Yu M, Ma J, Li X (2011) Expression pattern of a Gram-negative bacteria-binding protein in early embryonic development of / Artemia sinica and after bacterial challenge. Dev Comp Immunol 35:35-3 CrossRef - 作者单位：Rui Zhang (1)
Feng Yao (1)
Cheng Cheng (1)
Yifei Chen (1)
Ying Lv (1)
Zengrong Li (1)
Na Zhao (1)
Ting Wang (1)
Wenting Xin (1)
Xiangyang Zou (2)
Lin Hou (1)
1. College of Life Sciences, Liaoning Normal University, No. 1, Liushu South Street, Ganjingzi District, Dalian, 116081, People’s Republic of China
2. Department of Biology, Dalian Medical University, Dalian, 116044, People’s Republic of China - ISSN：1573-4978
文摘
As-NUPR1, a stress-related protein, plays an important role in post-diapause during embryonic development in the brine shrimp Artemia sinica. In the present study, successful expression of As-NUPR1 from the cDNA sequence isolated from A. sinica was demonstrated using a prokaryotic expression system. The recombinant protein consisted of 132 amino acids with a molecular weight of 15?kDa, and a predicted isoelectric point of 7.17. As-NUPR1 polyclonal antibodies were prepared by immunization of Balb/c mice with purified recombinant As-NUPR1 protein as an antigen, and immunological studies were carried out. Expression of As-NUPR1 during different developmental stages of the embryo and in response to salinity stress was analyzed in A. sinica using Western blots. The experimental results showed that the expression of As-NUPR1 is widely distributed at different developmental stages in A. sinica, and there was no tissue or organ specificity. Expression of As-NUPR1 decreased gradually during the diapause termination stage of embryo development, after which there was a general increase in expression after breaking the shell. In addition, As-NUPR1 expression was highly upregulated under conditions of high salinity. These results suggest that the As-NUPR1 protein is a stress-related protein that plays a role in protecting embryos from high salt damage in different embryonic developmental stages, especially during the post-diapause period.