A high-frequency in vitro multiplication, micromorphological studies and ex vitro rooting of Cadaba fruticosa (L.) Druce (Bahuguni): a multipurpose endangered medicinal shrub

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• 作者：Deepika Lodha ; Ashok Kumar Patel…
• 关键词：Cadaba fruticosa ; Cadabicine ; Endangered plant ; Ex vitro rooting ; Micropropagation ; Modified MS medium
• 刊名：Physiology and Molecular Biology of Plants
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：21
• 期：3
• 页码：407-415
• 全文大小：1,292 KB
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• 作者单位：Deepika Lodha (1)
  Ashok Kumar Patel (1)
  N. S. Shekhawat (1)
  
  1. Biotechnology Unit, Department of Botany (UGC -Centre for Advanced Study), Jai Narain Vyas University, New Campus, Prof. V. V. John Road, Jodhpur, 342 001, Rajasthan, India
  
• 刊物主题：Plant Sciences; Plant Physiology; Biophysics and Biological Physics; Cell Biology;
• 出版者：Springer India
• ISSN：0974-0430

文摘

An efficient and reproducible in vitro propagation protocol has been established for Cadaba fruticosa (L.) Druce. Surface-sterilized nodal stem segments of mature plant were used as explants for culture establishment. Multiple shoots were optimally differentiated from the nodal stem explants through bud breaking on Murashige and Skoog (1962) medium containing 3.0?mg l? benzyladenine (BA). The effect of different plant growth regulators and minerals were studied on different stages of micropropagation procedure (i.e., explant establishment, shoot multiplication/growth and ex vitro rooting). Additionally, for enhancing shoot multiplication during subculture, MS medium was modified (MMS) with higher levels of magnesium, potassium and sulphate ions. Out of these, MMS3 medium containing 0.25?mg l? each of BA and Kin (N6-furfuryladenine), with 0.1?mg l? NAA (α-naphthalene acetic acid) was found the best for shoot multiplication (42.45?±-.82 per culture vessel). The in vitro regenerated shoots were rooted under ex vitro conditions on treating the shoot base with 500?mg l? of IBA (indole-3 butyric acid) for 3?min on sterile Soilrite?. The ex vitro rooted plants were hardened in the greenhouse and transferred to the field with ?5?% survival rate. There were not any visual differences between wild and micropropagated plants in the field, although the later underwent significant changes during acclimatization. Micromorphological changes on leaf surface characters from in vitro to acclimatized plantlets were studied in terms of development of glandular trichomes, changes in vein spacing and vein structure in order to understand the nature of plant responses towards environmental conditions. The method developed and defined can be applied for commercial cultivation, which may be important for extraction of bioactive compounds and may facilitate conservation of this multipurpose endangered medicinal shrub.