A fast and robust protocol for metataxonomic analysis using RNAseq data
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- 作者：Jeremy W. Cox ; Richard A. Ballweg ; Diana H. Taft ; Prakash Velayutham…
- 关键词：Microbiome ; Metagenome ; Metatranscriptome ; Metataxonomics ; RNAseq ; Assembly of shotgun reads ; Altered Schaedler flora
- 刊名：Microbiome
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：5
- 期：1
- 全文大小：1068KB
- 刊物主题：Medical Microbiology; Bioinformatics; Microbial Ecology; Microbiology; Microbial Genetics and Genomics; Virology;
- 出版者：BioMed Central
- ISSN：2049-2618
- 卷排序：5
文摘
BackgroundMetagenomics is a rapidly emerging field aimed to analyze microbial diversity and dynamics by studying the genomic content of the microbiota. Metataxonomics tools analyze high-throughput sequencing data, primarily from 16S rRNA gene sequencing and DNAseq, to identify microorganisms and viruses within a complex mixture. With the growing demand for analysis of the functional microbiome, metatranscriptome studies attract more interest. To make metatranscriptomic data sufficient for metataxonomics, new analytical workflows are needed to deal with sparse and taxonomically less informative sequencing data.