Ultrasonic assessment of exercise-induced change in skeletal muscle glycogen content

详细信息    查看全文

• 作者：David C Nieman ; R Andrew Shanely…
• 关键词：Cycling ; Muscle biopsy ; Vastus lateralis ; Skeletal muscle ; Sonography
• 刊名：BMC Sports Science, Medicine and Rehabilitation
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：7
• 期：1
• 全文大小：1,838 KB
• 参考文献：1.Conlee RK. Muscle glycogen and exercise endurance: a twenty-year perspective. Exerc Sport Sci Rev. 1987;15:1-8.View Article PubMed
  2.Nieman DC, Davis JM, Henson DA, Gross SJ, Dumke CL, Utter AC, et al. Muscle cytokine mRNA changes after 2.5?h of cycling: influence of carbohydrate. Med Sci Sports Exerc. 2005;37:1283-0.View Article PubMed
  3.Nieman DC, Davis JM, Henson DA, Walberg-Rankin J, Shute M, Dumke CL, et al. Carbohydrate ingestion influences skeletal muscle cytokine mRNA and plasma cytokine levels after a 3-h run. J Appl Physiol. 2003;94:1917-5.View Article PubMed
  4.Avison MJ, Rothman DL, Nadel E, Shulman RG. Detection of human muscle glycogen by natural abundance 13C NMR. Proc Natl Acad Sci U S A. 1988;85:1634-.View Article PubMed Central PubMed
  5.Van Zijl PC, Jones CK, Ren J, Malloy CR, Sherry AD. MRI detection of glycogen in vivo by using chemical exchange saturation transfer imaging (glycoCEST). Proc Natl Acad Sci U S A. 2007;104:4359-4.View Article PubMed Central PubMed
  6.Kogan F, Hariharan H, Reddy R. Chemical Exchange Saturation Transfer (CEST) Imaging: Description of technique and potential clinical applications. Curr Radiol Rep. 2013;1:102-4.View Article PubMed Central PubMed
  7.Sikdar S, Wei Q, Cortes N. Dynamic ultrasound imaging applications to quantify musculoskeletal function. Exerc Sport Sci Rev. 2014;42:126-5.View Article PubMed
  8.Yim ES, Corrado G. Ultrasound in sports medicine: relevance of emerging techniques to clinical care of athletes. Sports Med. 2012;42:665-0.View Article PubMed
  9.Lopata RG, van Dijk JP, Pillen S, Nillesen MM, Maas H, Thijssen JM, et al. Dynamic imaging of skeletal muscle contraction in three orthogonal directions. J Appl Physiol. 2010;109:906-5.View Article PubMed
  10.Sarvazyan A, Tatarinov A, Sarvazyan N. Ultrasonic assessment of tissue hydration status. Ultrasonics. 2005;43:661-1.View Article PubMed
  11.Topchyan A, Tatarinov A, Sarvazyan N, Sarvazyan A. Ultrasound velocity in human muscle in vivo: perspective for edema studies. Ultrasonics. 2006;44:259-4.View Article PubMed
  12.Arts IM, Pillen S, Schelhaas HJ, Overeem S, Zwarts MJ. Normal values for quantitative muscle ultrasonography in adults. Muscle Nerve. 2010;41:32-1.View Article PubMed
  13.Hill JC, Millán IS. Validation of musculoskeletal ultrasound to assess and quantify muscle glycogen content. A novel approach Phys Sportsmed. 2014;42(3):45-2.View Article
  14.Shanely RA, Zwetsloot KA, Triplett NT, Meaney MP, Farris GE, Nieman DC. Human skeletal muscle biopsy procedures using the modified Bergstr?m technique. J Vis Exp. 2014: (91). doi: 10.3791/51812
  15.Kim CK, Bangsbo J, Strange S, Karpakka J, Saltin B. Metabolic response and muscle glycogen depletion pattern during prolonged electrically induced dynamic exercise in man. Scand J Rehabil Med. 1995;27:51-.PubMed
  16.Sherman WM, Doyle JA, Lamb DR, Strauss RH. Dietary carbohydrate, muscle glycogen, and exercise performance during 7 d of training. Am J Clin Nutr. 1993;57:27-1.PubMed
  17.Duhamel TA, Green HJ, Stewart RD, Foley KP, Smith IC, Ouyang J. Muscle metabolic, SR Ca(2+) -cycling responses to prolonged cycling, with and without glucose supplementation. J Appl Physiol. 2007;103:1986-8.View Article PubMed
  18.Lewis SF, Haller RG. The pathophysiology of McArdle’s disease: clues to regulation in exercise and fatigue. J Appl Physiol. 1986;61:391-01.PubMed
  19.Utter AC, McAnulty SR, Sarvazyan A, Query MC, Landram MJ. Evaluation of ultrasound velocity to assess the hydration status of wrestlers. J Strength Cond Res. 2010;24:1451-.View Article PubMed
  
• 作者单位：David C Nieman (1)
  R Andrew Shanely (2)
  Kevin A Zwetsloot (2)
  Mary Pat Meaney (1)
  Gerald E Farris (3)
  
  1. Appalachian State University, Human Performance Lab, North Carolina Research Campus, 600 Laureate Way, Kannapolis, NC, 28081, USA
  2. Department of Health and Exercise Science, Appalachian State University, Boone, NC, USA
  3. Department of Emergency Medicine, Carolinas Medical Center NorthEast, Concord, NC, USA
  
• 刊物主题：Sports Medicine; Orthopedics; Rehabilitation Medicine;
• 出版者：BioMed Central
• ISSN：2052-1847

文摘

Background Ultrasound imaging is a valuable tool in exercise and sport science research, and has been used to visualize and track real-time movement of muscles and tendons, estimate hydration status in body tissues, and most recently, quantify skeletal muscle glycogen content. In this validation study, direct glycogen quantification from pre-and post-exercise muscle biopsy samples was compared with glycogen content estimates made through a portable, diagnostic high-frequency ultrasound and cloud-based software system (MuscleSound?, Denver, CO). Methods Well-trained cyclists (N--0, age 38.4?±-.0 y, 351?±-7.6 watts_max) participated in a 75-km cycling time trial on their own bicycles using CompuTrainer Pro Model 8001 trainers (RacerMate, Seattle, WA). Muscle biopsy samples and ultrasound measurements were acquired pre- and post-exercise. Specific locations on the vastus lateralis were marked, and a trained technician used a 12?MHz linear transducer and a standard diagnostic high resolution GE LOGIQ-e ultrasound machine (GE Healthcare, Milwaukee, WI) to make three ultrasound measurements. Ultrasound images were pre-processed to isolate the muscle area under analysis, with the mean pixel intensity averaged from the three scans and scaled (0 to 100 scale) to create the glycogen score. Pre- and post-exercise muscle biopsy samples were acquired at the vastus lateralis location (2?cm apart) using the suction-modified percutaneous needle biopsy procedure, and analyzed for glycogen content. Results The 20 cyclists completed the 75-km cycling time trial in 168?±-6.0?minutes at a power output of 193?±-7.8 watts (54.2?±-.6% watts_max). Muscle glycogen decreased 77.2?±-7.4%, with an absolute change of 71.4?±-3.1?mmol glycogen per kilogram of muscle. The MuscleSound? change score at the vastus lateralis site correlated highly with change in measured muscle glycogen content (R--.92, P-lt;-.001). Conclusions MuscleSound? change scores acquired from an average of three ultrasound scans at the vastus lateralis site correlated significantly with change in vastus lateralis muscle glycogen content. These data support the use of the MuscleSound? system for accurately and non-invasively estimating exercise-induced decreases in vastus lateralis skeletal muscle glycogen content.