Gametic embryogenesis through isolated microspore culture in Corylus avellana L.

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• 作者：Marines Marli Gniech Karasawa…
• 关键词：Haploid ; Hazelnut ; Microspore ; derived embryos ; Temperature stress
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：124
• 期：3
• 页码：635-647
• 全文大小：1,652 KB
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• 作者单位：Marines Marli Gniech Karasawa (1)
  Benedetta Chiancone (2)
  Valeria Gianguzzi (1)
  Ahmed Mohamed Abdelgalel (1)
  Roberto Botta (3)
  Chiara Sartor (3)
  Maria Antonietta Germanà (1)
  
  1. Dipartimento di Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale Delle Scienze, 11 Ed. 4, 90128, Palermo, Italy
  2. Dipartimento di Scienze degli Alimenti, Università degli Studi di Parma, Parma, Italy
  3. Dipartimento di Scienze Agrarie, Forestali e Alimentari – DISAFA, Università degli Studi di Torino, L.go P. Braccini 2, 10095, Grugliasco, TO, Italy
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report of early embryos being recovered from the five hazelnut genotypes via isolated microspore culture. Analysis of the embryo genotypes using SSR markers demonstrated the embryos had a single allele per each locus whereas the parent cultivar was heterozygous, indicating they developed from haploid microspores. The response to the culture treatments, was, however, genotype-dependent, as previously reported for male gamete embryogenesis in other fruit crops. Keywords Haploid Hazelnut Microspore-derived embryos Temperature stress