Timosaponin B-II ameliorates scopolamine-induced cognition deficits by attenuating acetylcholinesterase activity and brain oxidative damage in mice
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- 作者：Xu Zhao ; Chunmei Liu ; Yu Qi ; Lina Fang ; Jie Luo ; Kaishun Bi…
- 关键词：Timosaponin B ; II ; Alzheimer’s disease ; Learning and memory ; Acetylcholinesterase ; Oxidative stress
- 刊名：Metabolic Brain Disease
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：31
- 期：6
- 页码：1455-1461
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Neurosciences; Neurology; Metabolic Diseases; Biochemistry, general; Oncology;
- 出版者：Springer US
- ISSN：1573-7365
- 卷排序：31
文摘
Timosaponin B-II (TB-II) is a main active saponin isolated from the rhizome of Anemarrhena asphodeloides Bge., which is widely used in traditional Chinese medicine. In this study, the effect of TB-II on learning and memory was investigated in a scopolamine-induced mouse model of Alzheimer’s disease. The results of behavioral tests indicated that TB-II significantly increased the spontaneous alternation in the Y-maze test, and reversed the shortening of step-through latency induced by scopolamine in the passive avoidance test, showing protective effects on short-term and working memory. In the Morris water maze test, TB-II reduced the escape latency time in the training trial, and increased the swimming time in the target quadrant in the probe trial. Biochemical data demonstrated that TB-II significantly inhibited acetylcholinesterase (AChE) activity in the cerebral cortex and hippocampus of mice. Moreover, TB-II markably attenuated the reduction in glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities, and decreased malondialdehyde (MDA) levels, which are key biomarkers of brain oxidative stress. These results indicated that TB-II offers protection against scopolamine-induced deficits in learning and memory, possibly by inhibiting AChE and preventing oxidative stress damage. The findings suggested that TB-II has a potential therapeutic effect on cognitive and behavioral impairment.